抗鸡传染性支气管炎病毒N蛋白单克隆抗体制备与鉴定  

Preparation and characterization of the monoclonal antibodies against nucleocapsid protein of IBV

作　　者：于丹[1,2] 韩宗玺[1] 邵昱昊[1] 李慧昕[1] 金鑫[2] 孔宪刚[1] 刘胜旺[1] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/禽传染病研究室,黑龙江哈尔滨150001 [2]延边大学农学院,吉林延边134000

出　　处：《中国预防兽医学报》2009年第8期654-656,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：十一五国家科技支撑计划(006BAD6A03)

摘　　要：为建立抗鸡传染性支气管炎病毒(IBV)的单克隆抗体(MAb),利用浓缩的IBV致弱株CK/CH/LDL97ⅠF115病毒免疫BALB/c小鼠,采用杂交瘤技术,经间接ELISA和有限稀释法,经筛选和克隆后获得了一株抗IBV N蛋白MAb的杂交瘤细胞系(2D2)。经鉴定,其MAb的重链为IgG1亚型,轻链为κ链。ELISA和western blot试验结果表明,所获得的这株2D2 MAb可特异性识别IBV N蛋白。2D2 MAb可与多种血清型IBV发生反应,表明该MAb识别的表位可能位于N蛋白的保守区域。为进一步鉴定IBV的表位及诊断试剂的研究奠定了基础。Monoclonal antibody （MAb） hybridoma cell lines 2D2 that secreted MAb against N protein of avian infectious bronchitis virus were developed by fusion myeloma cells with spleen cells of BALB/c mouse immunized with 1BV CK/CH/LDL97 F115 virus. The MAb 2D2 belonged to IgGt subtype with K chain. ELISA and western blot assays showed that MAb 2D2 reacted specifically with N protein of IBV. The MAb 2D2 can also react with N protein of several different IBV serotypes, indicating that the epitope of the 2D2 may locate in the conserved region of IBV. This MAb provided a foundation for study of genetic variation of IBV and for the development of diagnostic reagent.

关 键 词：传染性支气管炎病毒 N蛋白 单克隆抗体 

分 类 号：S853.3[农业科学—临床兽医学]