通心络胶囊对载脂蛋白E基因敲除小鼠主动脉粥样硬化的影响  

Effects of Tongxinluo Capsule on Aorta Atherosclerosis of Apolipoprotein E-knockout Mice

作　　者：韩旭[1] 李七一[1] 夏卫军[1] 赖仁胜[1] 陈小虎[1] 郭宏敏[1] 赵惠[1] 

机构地区：[1]江苏省中医院,南京210029

出　　处：《中国中医急症》2009年第8期1292-1294,共3页Journal of Emergency in Traditional Chinese Medicine

基　　金：江苏省自然科学基金项目(No.BK2006157)

摘　　要：目的观察通心络胶囊对载脂蛋白E基因敲除[ApoE(-/-)]小鼠主动脉斑块病理组织学的干预变化,探究其治疗主动脉粥样硬化的机制。方法将40只ApoE(-/-)小鼠建立主动脉粥样硬化模型,随机分为模型组、辛伐他汀组和通心络胶囊低、中高剂量组,各治疗组分别给予辛伐他汀及高、中、低剂量的通心络胶囊进行干预8周;另选8只同系的小鼠作为正常对照组。通过对模型组主动脉斑块病理组织学指标变化的观察,比较分析通心络胶囊对ApoE(-/-)小鼠主动脉粥样硬化的调节作用和机制。结果模型组所有小鼠主动脉病变主要为内膜增厚,内皮下有多量脂质沉积,几乎绕动脉壁全周,但厚薄不等,主动脉粥样硬化性病变评分为(3.0±1.07)分;辛伐他汀组较模型组减轻,病变评分为(1.38±1.51)分;通心络胶囊低剂量组病变评分为(2.0±1.31)分,中剂量组病变评分为(1.63±1.41)分,高剂量组病变评分为(1.5±1.41)分。通心络胶囊中、高剂量组与模型组差异显著;与辛伐他汀组相近。结论通心络胶囊对ApoE(-/-)小鼠主动脉粥样硬化的形成有一定的抑制作用,其中以高剂量组效果最好,但中、高剂量组与辛伐他汀组作用相当。Objective： To explore the therapeutic mechanism of Tongxinluo Capsule on aorta atherosclerosis of apolipoprotein E - knockedout [ApoE （ - / - ） ] mice. Methods： 40 ApoE （ - / - ） model mice were randomly divided into control group, simvastatin group, Tongxinluo Capsule low - dose, middle - dose and high - dose group. Each group was given simvastatin, low - dose, middle - dose and high - dose of Tongxinluo Capulses during 8 weeks. Then, 8 syngenic mice were allocated into normal group. Through observing the change of histopathology indices in aorta atherosclerosis, the adjustable mechanism of Tongxinluo Capsule in aorta atherosclerosis of ApoE（ - / - ） mice were compared. Results： The aorta processes of mice in the model group were endomembrane thickening and much lipidoses infer -endomembrane. The aorta process score was （3.0 ± 1.07）. Pathological changes in the simvastatin group were less than those of model group, and the score was （ 1.38 ± 1.51 ） . The score of the Tongxinluo Capsule low - dose, middle - dose and high - dose group was respectively （2.0 ± 1.31 ）, （ 1.63 ± 1.41 ） and （ 1.5± 1.41 ） . Significant differences were found between Tongxinluo Capsule middle - dose, high - dose group and model group. But no significant difference was found between Tongxinluo Capsule middle-dose, high-dose group and simvastatin group. Conclusion： Tongxinluo Capsule could inhibit aorta atherosclerosis formation of ApoE （ - / - ） mice. The therapeutic effect of high - dose group is better than low - dose and middle - dose group. The effects of high - dose and middle - dose group are equivalent with that of the simvastatin group.

关 键 词：ApoE(-/-)小鼠 主动脉粥样硬化 干预作用 通心络胶囊 

分 类 号：R285.5[医药卫生—中药学]