水稻HL-CMS中2个雄性不育候选基因表达模式的初步研究  

Expression Pattern of Two Male Sterile Candidate Genes in Honglian Cytoplasmic Male Sterile Rice Line

作　　者：陈为[1] 周杰[1] 谭艳平[1] 程钢[1] 刘新琼[1] 王春台[1] 刘学群[1] 

机构地区：[1]中南民族大学生命科学学院/生物技术国家民委重点实验室,武汉430074

出　　处：《华中农业大学学报》2009年第4期394-397,共4页Journal of Huazhong Agricultural University

基　　金：国家"863"高技术研究发展专项(2008AA10Z118);国家自然科学基金项目(30771163)资助

摘　　要：采用实时荧光定量PCR技术,比较了水稻红莲型细胞质雄性不育(HL-CMS)不育系粤泰A(YTA)中的2个雄性不育候选基因HLsp和orfH79在根、茎、叶以及减数分裂期小穗和三核期花药中的表达模式。结果表明,HLsp和orfH79均在减数分裂期小穗中表达最高,而在营养生长的根、茎、叶及成熟花粉中表达很低。HLsp在粤泰A不同组织中的表达均低于orfH79,但HLsp在减数分裂期小穗中的表达量与在其它组织中的表达量的差异明显比orfH79大。2个不育候选基因HLsp和orfH79在HL-CMS不育系YTA不同组织及发育时期的表达差异,暗示二者在水稻HL-CMS的发生中可能行使不同的功能。The expression of two male sterile candidate genes, HLsp and orfH79, in root, stem, leaf,meiosis spikelet,and trinucleate pollen of rice HongLian cytoplasmic male sterile（HL-CMS） sterile line,YTA,were studied with real-time PCR. The results showed that the expression of both HLsp and orfH79 were higher in meiosis spikelet than that in the root, stem, leaf, and trinucleate pollen. The expression level of HLsp was lower than that of orfH79 in different tissues of YTA, but the expression difference of HLsp between meiosis spikelet and other tissues was higher than that of orfH79. This difference maybe indicate that HLsp and orfH79 play a different role in the abortion process of rice HL- CMS pollen.

关 键 词：水稻 红莲型细胞质雄性不育 实时荧光定量PCR 基因表达 

分 类 号：S511.024[农业科学—作物学]