十字划线与三维试验法检测革兰阴性杆菌产AmpC酶的比较  

Comparison of Detecting AmpC Produced by Gram-negative Bacilli with Modified Three-dimensional Extract Test and Three-dimensional Test

作　　者：苏丹虹[1] 谭嘉庆[2] 管婧[3] 

机构地区：[1]广州医学院第一附属医院检验科,广州510120 [2]中山大学附属第一医院肾病研究室,广州510800 [3]广州医学院,广州510000

出　　处：《现代检验医学杂志》2009年第4期74-77,共4页Journal of Modern Laboratory Medicine

基　　金：基金项目：广州市科技局重大攻关项目资助课题（z00621-E0141）,广州医学院资助课题.

摘　　要：目的建立一种简便可行的检测革兰阴性杆菌产AmpC酶的方法，并与三维试验比较。方法采用新建立的十字划线法，以三维试验为参考方法，对45株临床标本分离的革兰阴性杆菌产AmpC酶进行检测，用阴沟肠杆菌ATCC700323作阴性对照；阴沟肠杆菌029M产AmpC酶株作阳性对照，并以三维试验比较检测结果。结果十字划线和三维试验对45株革兰阴性杆菌作AmpC酶的检测，其两种方法检出率分别为35．6％（16／45）和22．2％（10／45），两者之间差异有统计学显著性意义（酽=13．72，P〈0．05），与三维试验比较十字划线假阳性7株、假阴性1株，准确度为82．2％，敏感度为90％，特异度为80％。结论十字划线法可作为临床检测革兰阴性杆菌产AmpC酶较为理想的筛查方法。Objective To establish a simple and workable method of detecting AmpC β-lactamase from Gram-negative bacil- li. Methods AmpC from 45 Gram-negative isolates were measured by modified three-dimensional extract test and the re- sult was compared with three-dimensional test. E. cloacae ATCC700323 was used as negative control,and E. cloacae O29M was used as positive control. Results Conducted by modified three-dimensional extract test and three-dimensional test,45 isolates were tested and AmpC was found positive as 35.6 % （16/45） and 22.2% （10/45） respectively. There was the re- markable difference between two methods （x2=13.72,P〈0. 05）. Compared with three-dimensional test,the concordance, sensitivities and specificities result of modified three-dimensional extract test were 82.2%, 80. 0% and 90. 0% respective- ly. Conclusion Modified three-dimensional extract test is a simple and adaptable method of clinical detecting to AmpC from Gram-negative bacilli.

关 键 词：AMPC酶 三维试验 十字划线法 

分 类 号：R446.5[医药卫生—诊断学]