中国襄樊地区部分慢性乙型肝炎患者HBV基因型分析  

Hepatitis B Virus Genotypes in Chronic Liver Disease Patients from Xiangfan in China

作　　者：马亚平[1] 王军梅[1] 曹红[1] 徐天骄[2] 

机构地区：[1]湖北省襄樊市中心医院检验科,湖北襄樊441021 [2]解放军第477医院肿瘤科,湖北襄樊441021

出　　处：《现代检验医学杂志》2009年第4期98-101,共4页Journal of Modern Laboratory Medicine

摘　　要：目的了解中国湖北省襄樊地区乙型肝炎患者外周血中病毒基因型分布特点，并考察不同基因型与临床的相关性。方法使用PCR-RDB法检测143例慢性乙型肝炎患者的HBV基因型。结果在襄樊地区143例慢性乙型肝炎患者中发现了四种HBV基因型，分别是B，C，D和B＋C，其中B型52例（36．36％），C型82例（57．34％），D型3例（2．10％），B＋C型6例（4．20％）。在乙型肝炎无症状携带者（ASC）和慢性乙型肝炎（CHB）组中，基因型B和基因型C所占比例之间差异无统计学意义（x2=0．04，0．04，P〉0．05），在慢性重症肝炎（CLF），乙肝后肝硬化（LC）和原发性肝细胞癌（HCC）组中，基因型C的比例明显多于基因型B（X2=10．28，23．75，27．59，P〈0．01）。结论慢性乙型肝炎患者中B型和C型是地区慢性乙型肝炎患者HBV的优势基因型。结果揭示基因型C与HBV感染的严重程度相关。Objective To study the Hepatitis B Virus （HBV） genotypes and their effect on the progression and outcome in patients with chronic liver diseases from Xiangfan in China. Methods Sera from 143 HBV-related chronic liver disease cas- es were tested for HBV genotype using Polymerase Chain Reaction-Reverse Dot Blot （PCR-RDB）. Results Genotypes B, C,D and B＋C were present in all CLDB patient categories. The genotype distribution for the 143 patients with CLDB was as follows ：genotype B 52/143 （36.36%）;genotype C 82/143（57. 34%）,genotype D 3/143（2. 10%）,mixed-genotype B＋C 6/143 （4. 20%）. In ASC and CHB groups ,the distribution of genotype 13 and C showed no significant difference （x2= 0. 04, 0. 04,P〉0.05）,respectively. The ratio of genotype C in the CLF,LC and HCC groups was obviously higher than that genotype B（x2=10. 28,23.75,27.59,P〈0. 01） ,respectively. Conclusion Genotypes B,C,D and B＋C were present in all CLDB patient categories from Xiangfan,China. Genotype B and C is the major genotype and is prevalent in all patient cate- gories. The results indicated that genotype C is associated with the development of severe liver disease.

关 键 词：乙型肝炎 基因型 

分 类 号：R512.62[医药卫生—内科学] Q781[医药卫生—临床医学]