UV-B辐射对拟南芥细胞周期G1/S期转变的影响  

UV-B Induced G1/S Arrest in Arabidopsis Root Tips

作　　者：王静[1] 蒋磊[1] 王艳[1,2] 李韶山[1] 

机构地区：[1]华南师范大学生命科学学院,广东省高等学校生态与环境科学重点实验室,广州510631 [2]暨南大学理工学院,广州510632

出　　处：《植物学报》2009年第4期426-433,共8页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.30670156和No.30470283);高等学校博士学科点专项科研基金(No.20070574003);教育部留学回国人员科研启动基金(No.2005-383)

摘　　要：以同步化的拟南芥(Arabidopsis thaliana)根尖细胞为材料,研究了UV-B辐射对拟南芥细胞周期G1/S期转变的影响。细胞周期荧光显微图像分析表明,UV-B辐射延缓了拟南芥根尖细胞G1/S期的转变。基因表达的RT-PCR检测表明,在UV-B辐射下G1/S期转变的标志基因Histone H4和E2Fa的表达受抑制,而G1/S期转变的抑制因子KRP2基因表达则受诱导上调。单细胞凝胶电泳检测结果表明,UV-B辐射引起拟南芥根尖细胞内积累大量的环丁烷嘧啶二聚体。以上结果表明,UV-B辐射抑制植物的生长可能受细胞周期和DNA损伤调控。Cell cycle regulation is closely linked to plant growth and development. The plant cell cycle responds rapidly to stimuli from both the internal and external environment. We present a model of partially synchronized root tips of Arabidopsis to study the effects of UV-B radiation on the G1/S transition in the cell cycle. Analysis of the fluorescence image under microscopy indicated that G1/S arrest was induced by UV-B radiation. The expression of two factors of GI/S transition, Histone H4 and E2Fa, was repressed under UV-B radiation, whereas the negative factor of G1/S transition, KRP2, was upregulated by UV-B radiation. UV-B radiation during the cell cycle induced accumulation of cyclobutane pyrimidine dimers. All these results suggested that UV-B- induced plant growth inhibition may be mediated by cell cycle regulation and DNA damage.

关 键 词：拟南芥 细胞周期 基因表达 G1/S期转变 根尖 单细胞凝胶电泳 UV-B辐射 

分 类 号：Q942[生物学—植物学]