早期激素性股骨头坏死骨细胞凋亡与bcl-2和p53表达的实验研究  

Study on steroid-induced avascular necrosis of femoral head in the early days and apoptosis and the experimeantal study on bcl-2 and p53

作　　者：张立岩[1] 马恩元[1] 祖德玉[1] 王大麟[1] 

机构地区：[1]北华大学附属医院骨外1科,吉林吉林132011

出　　处：《中国现代医学杂志》2009年第13期1945-1947,1951,共4页China Journal of Modern Medicine

基　　金：吉林省吉林市科学技术发展计划基金项目(No:2008037)

摘　　要：目的探讨细胞凋亡及其调控基因在早期激素性股骨头坏死中发挥的作用。方法健康兔30只,将实验动物随机分为实验组15只,对照组15只。模型成功后,进行组织形态学、骨细胞凋亡、免疫组织化学染色检测。结果光镜下实验组较对照组骨小梁变细、稀疏,细胞核变形,骨细胞少见,空骨陷窝明显增多。实验组凋亡细胞明显多于对照组,bcl-2阳性细胞比率低于对照组,p53阳性细胞比率高于对照组。结论早期激素性股骨头坏死是骨细胞坏死和骨细胞凋亡共同作用的结果,大剂量激素抑制了bcl-2蛋白的表达,促进了p53蛋白的表达,bcl-2与p53共同调控骨细胞的凋亡。[Objective] To study the apoptosis and the function played by the steroid-induced avascular necrosis of femoral head＇s controlling genes in the early days. [Methods] 30 healthy rabbits were divided into 2 groups, 15 for experimental group and 15 for control group. Test the histomorphology, apoptosis, immunohistochemistry after the models are finished. [Results] Observation under microscope compared with the control group, bone trabecula in experimental group becomes skarce, and empty bone lacune increased. Much more apoptosis emerged in experimental group, more than in control group. Bcl-2： the rate of masculine cells is lower than that of control group, p53： the rate of masculine cells is much higher than that of control group. [Conclusions] The death of bone cells and apoptosis lead to the steroid-induced avascular necrosis of femoral head in the early days. The proteinum bel-2 is restrained by too much hormone, while the hormone advances proteinum p53, so proteinum bel-2 and proteinum p53 both control the apoptosis.

关 键 词：激素 股骨头坏死 细胞凋亡 调控 

分 类 号：R681[医药卫生—骨科学] R-332[医药卫生—外科学]