c-Kit和n-NOS2双重染色在豚鼠膀胱Cajal样间质细胞的表达  

Expressions of c-kit and n-NOS2 in adult guinea-pig's bladder cells of Cajal

作　　者：纪世琪[1] 王勤章[1] 丁国富[1] 朱国栋[1] 

机构地区：[1]石河子大学医学院第一附属医院,新疆石河子832008

出　　处：《中国现代医学杂志》2009年第13期1980-1981,1985,共3页China Journal of Modern Medicine

基　　金：国家自然科学基金(No:30860281)

摘　　要：提要:目的研究c-Kit与n-NOS2在豚鼠膀胱Cajal样间质细胞的共表达情况。方法采用培养成年豚鼠膀胱Cajal样间质细胞,c-Kit免疫荧光和n-NOS2免疫荧光双重染色。结果豚鼠膀胱内Cajal样间质细胞体为梭形或卵圆形,常见2～3个细长的突起。c-Kit和n-NOS2免疫荧光双重染色发现两种抗体在Cajal样间质细胞共表达。结论本研究结果提示在豚鼠膀胱Cajal样间质细胞内c-Kit和n-NOS2共存,Cajal样间质细胞具备产生NO的功能,Cajal样间质细胞存在调节膀胱自律活动的结构基础。[ Objective ] To study the distribution of c-Kit and n-NOS2 in adult guinea-pig＇s bladder cells of Cajal. [Methods] Bladder cells of Cajal from guinea-pigs were examined for detection of c-Kit and n-NOS2 by immunocytochemistry. [Result] The ICCs were seen in adult guinea-pig＇s bladder. The ceils were spindle shape cells with two slender processes. The c-Kit and n-NOS2 were both expressed in adult guinea-pig＇s bladder cells of Cajal. [Conclusion] Our results showed that c-Kit and n-NOS2 were both existing in bladder ICC-like cells. Bladder ICCs may produce NO. The ICCs may be involved in the regulation of the auto-rhythm movement of bladder.

关 键 词：CAJAL样间质细胞 酪氨酸激酶受体(c—Kit) 一氧化氮合酶2(n—NOS2) 免疫荧光双染 

分 类 号：R392.31[医药卫生—免疫学]