基于多壁碳纳米管修饰电极的保泰松的电致化学发光检测  

Electrochemiluminescence Determination of Phenylbutazone Based on Modified Electrode by Multi-wall Carbon Nanotube

作　　者：陈毅挺[1,2] 何伟文[1,2] 林棋[1] 娄本勇[1] 陈国南[2] 

机构地区：[1]闽江学院化学与化学工程系,福州350108 [2]福州大学食品安全与检测技术教育部重点实验室,福州350002

出　　处：《化学学报》2009年第14期1615-1620,共6页Acta Chimica Sinica

基　　金：国家自然科学基金(Nos.20735002;20575011);福建省自然科学基金(No.2008J0164);福建省青年科技人才创新项目(No.2008F3081);福建省教育厅科技项目(Nos.JB07168);食品安全分析与检测技术教育部重点实验室(福州大学)开放项目(No.FS06004);福州市科技项目(No.2007-Z-81)资助项目

摘　　要：基于保泰松对联吡啶钌的电致化学发光信号有较强的增敏作用,使用多壁碳纳米管修饰玻碳电极作为工作电极,建立了一种保泰松的电致化学发光检测方法.对测定条件进行了一系列优化,结果表明,当发光试剂联吡啶钌的浓度为1.0×10-5mol/L时,在0.05mol/LpH10.20的Na2HPO4-NaOH介质中保泰松对联吡啶钌电化学发光信号的增敏效果最强.在此条件下,保泰松的线性范围为0.5～200μmol/L,检测限(S/N=3)为0.2μmol/L,与使用未经修饰的裸玻碳电极相比,检测限下降了约一个数量级.该检测方法还被用于加标人血清样品中保泰松的测定,回收率在85.3%～95.0%之间,结果令人满意,可望用于保泰松的药物临床分析与质量监控.Phenylbutazone （PB） was found to be able to enhance the electrochemiluminescence （ECL） of Ru（bpy）^2＋ system, based on which, a novel ECL method for the determination of PB at a multi-wall carbon nanotube （MWNT） modified glassy carbon electrode （GCE） was developed. The effect of enhanced ECL by PB was highest in the 0.05 mol/L pH 10.20 Na2HPO4-NaOH buffer with 1.0×10^-5mol/L Ru（bpy）^2＋ . Under the optimum conditions, the enhanced ECL intensity was linear with PB concentration in the range of 0.5-200 μmol/L. The detection limit （defined as S/N=3） was 0.2 μmol/L, which is lower than that on a bare GCE by one order of magnitude. The method was successfully applied to the determination of PB in a spiked human serum sample, and the results were satisfactory with the recoveries of 85.3%-95.0%. The developed detection method for PB is hopeful to be applied to clinical drug analysis and quality control.

关 键 词：保泰松 联吡啶CT 电致化学发光 

分 类 号：O657.3[理学—分析化学]