健康人群中大肠埃希菌耐药整合子的检测  

Detection of resistance integron structure in E. coli isolates from healthy people

作　　者：李婷[1] 马平[1] 赵冰[1] 汤琰[1] 杨玲凤[1] 

机构地区：[1]上海市浦东新区疾病预防控制中心,上海200135

出　　处：《华西药学杂志》2009年第4期372-374,共3页West China Journal of Pharmaceutical Sciences

基　　金：上海市卫生局资助项目(044110)

摘　　要：目的了解上海市浦东新区健康人群中肠道大肠埃希菌的耐药情况,并检测其耐药整合子的携带情况和结构。方法采用纸片扩散法(E-B法)检测17种抗生素药物的敏感性;PCR法扩增1、2、3类整合酶,对1类整合酶阳性样本进一步扩增整合子;扩增产物经限制性内切酶分析测序后,分析其所携带的基因盒结构和可能的功能。结果浦东新区健康人群中肠道大肠埃希菌多重耐药率为63.5%,耐药性主要集中在老一代抗生素,对较新的抗生素仍然敏感。1、2类整合酶的检出率分别为38.5%、4%,且有1%同时检测到1、2类整合酶,未检测到3类整合酶。结论健康人群中肠道大肠埃希菌耐药现象已非常普遍,耐药整合子在人群中分布也比较广泛,已成为耐药基因的储存,但和临床相比还有差别。OBJECTIVE To investigate the level and characterization of Escherichia coli（E.coli）isolated from healthy people in Pudong new area of Shanghai and detect the distribution and structure of resistance integron.METHODS Antibiotics susceptibility of 17 antibiotics was tested by the disk diffusion method.The presence of class 1,class 2 and class 3 integrase were examined by PCR,and then the variable region between the conserved segment of class 1 integron was amplified.The integron content was characterized by restricted enzyme fragment length polymorphisms（RFLP） and sequenced.RESULTS The multiple resistance rate of E.coli isolated from healthy people in Pudong new area of Shanghai was 63.5%.But the resistance was mainly to older antibiotics.The newer antibiotics were still susceptible.Int 1 was detected in 38.5% of the isolates;int 2 was detected in 4% isolates,both int 1 and int 2 were detected in other 2（1%）isolates and no int 3 was detected.CONCLUSION Resistance in E.coli isolated from healthy people is common,and the resistance integrons also widely distribute in common people.But there are still differences between the clinical isolates.

关 键 词：健康人群 耐药性 整合酶 整合子 基因盒 

分 类 号：R917[医药卫生—药物分析学]