HPLC测定兔血浆中的藁本内酯  

Determination of ligustilide in rabbit plasma by HPLC

作　　者：赵惠茹[1] 周晓棉[2] 冯素香[3] 靖会[1] 

机构地区：[1]西安医学院药学系,陕西西安710021 [2]沈阳药科大学药学院,辽宁沈阳110016 [3]河南中医学院药学院,河南郑州450008

出　　处：《华西药学杂志》2009年第4期414-416,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的采用HPLC测定兔血浆中藁本内酯的浓度。方法样品经正己烷萃取后进样分析。色谱柱为Kromasil C18（150mm×4.6 mm,5μm）,流动相为甲醇-水（75：25）,流速1.0 ml.min-1,检测波长328 nm,测定兔灌胃100 mg.kg-1当归挥发油有效部位后的血药浓度变化。结果藁本内酯在血浆中的线性范围为0.2～6.4μg.ml^-1,最低检测浓度为0.1μg.ml^-1,日内和日间RSD均小于8%,平均回收率分别为97.18%、93.91%。兔灌胃后,Tpeak=1.28±0.12 h,Cmax=3.86±0.24μg.ml^-1,t1/2α=2.04±0.20 h。结论所建方法符合生物样品的测定要求,可用于兔血浆中藁本内酯的测定和药物动力学研究。OBJECTIVE To develope an HPLC method to assess the concentration of ligustilide in rabbit plasma.METHODS After oral administration of volatile oil at a dose of 100 mg·kg-1 to rabbit,blood samples were collected and extracted with n-hexane.The HPLC system consisted of C18（150 mm×4.6 mm,5 μm）column using MeOH-H2O（75：25） as mobile phase at a flow rate of 1.0 and detection at 328 nm.RESULTS Linear calibration was obtained over a concentration range of 0.2-6.4 μg·ml^-1 for ligustilide.The minimum limit of detection was 0.1 μg·ml^-1.The inter-day and intra-day precision were less than 8% and the recovery of ligusitilide in plasma were 97.18% and 93.91%.Pharmacokinetic parameters were estimated as follows：Tpeak 1.28±0.12 h,Cmax 3.86±0.24 μg·ml^-1,t1/2α 2.04±0.20 h,respectively.CONCLUSION This method is suitable for the pharmacokinetic study of ligustilide in rabbit.

关 键 词：高效液相色谱法 藁本内酯 药物动力学 当归挥发油 

分 类 号：R917[医药卫生—药物分析学]