基于电化学方法设计的唾液葡萄糖检测仪  

Electrochemistry-based design of a detector for saliva glucose

作　　者：朱永丽[1] 赵秋生[1] 邵珠光[1] 景志伟[1] 

机构地区：[1]天津医科大学生物医学工程系,天津市300070

出　　处：《中国组织工程研究与临床康复》2009年第30期5915-5918,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：旨在设计一种基于电化学方法的唾液葡萄糖检测仪,根据糖尿病患者的唾液葡萄糖含量与血糖含量的相关性,通过检测糖尿病患者的唾液葡萄糖含量,实现糖尿病患者的无创病情监控。实验制备普鲁士蓝修饰电极作为三电极系统的工作电极,提高电极对H2O2的电催化还原反应的灵敏度,降低工作电压。利用三电极检测系统,检测在葡萄糖氧化酶催化作用下葡萄糖氧化还原反应产生的电流。采用屏蔽层驱动技术,避免检测到的微弱信号在传输过程中的衰减,从而达到准确测量。采用单片机C8051F020对整个系统进行控制,并且为电极提供扫描电压,对检测结果进行A/D转换等处理,最后将结果显示在液晶屏上。该检测仪工作电压为0.496V,检测灵敏度为0.1mmol/L;由该仪器检测的唾液葡萄糖浓度与精密分析仪蓝标LK98B型测得的结果只差一个比例常数。结果提示,该唾液葡萄糖检测仪具有工作电压低、灵敏度高、总体性能良好的特点,可以达到检测要求。A kind of saliva glucose detector based on electrochemistry is designed to measure the saliva glucese of diabetes mellitus, so as to achieve non-invasive measurement for diabetes mellitus state. A Prussian blue modified glassy carbon electrode was designed as the working electrode of the three-electrode system to improve the electrode sensitivity to H2O2＇s electrochemical reaction in a lower voltage. A three-electrode system was employed for measuring the current generated by the oxidation-reduction reaction of glucose catalyzed by glucose oxidase. Technique of shield-driving was used to avoid the attenuation of the voltage when it was processed, so as to ensure the precision measurement. A single chip microcomputer, C8051F020, was used to control the system and provide scanning voltage for the electrode, make A/D transition and process the result. Then the outputs were displayed on liquid crystal display. This detector＇s operating voltage was 0.496 V. The sensltivity was 0.1 mmol/L. There was a constant of proportionality between the measuring results of this detector and the precise analysator, Lanbiao LK98B. Results of the study show that this detector has low operating voltage, high sensitivity and good function of whole system, and can meet the request of measure.

关 键 词：唾液葡萄糖 普鲁士蓝 酶电极 单片机 

分 类 号：R318[医药卫生—生物医学工程]