超声微泡介导碱性成纤维细胞生长因子基因转染鼠缺血心肌的实验研究  

Experimental Study on Transferring bFGF into the Ischemic Myocardium of Rat Mediated by Microbubbles

作　　者：盛娓娓[1] 黄晶[1] 周大燕[1] 李文章[1] 刘地川[1] 邓昌明[1] 王志刚[2] 

机构地区：[1]重庆医科大学附属第二医院心内科,重庆市400010 [2]重庆医科大学附属第二医院超声影像研究所,重庆市400010

出　　处：《中国超声医学杂志》2009年第8期725-727,共3页Chinese Journal of Ultrasound in Medicine

基　　金：国家自然科学基金重点项目（No.30527001）及面上项目（No.30670870）,重庆市科技攻关项目（No.CSTS2005AA50085）

摘　　要：目的探讨超声激活携基因微泡对外源基因在梗死心肌组织中表达的影响及治疗性血管新生在鼠心梗模型中的可行性研究。 方法采用心肌内注射途径,超声激活携基因微泡组注射携碱性成纤维细胞生长因子（basic fibroblast growth factor,bFGF）基因微泡后进行超声辐照,单纯基因组和对照组分别注射等量bFGF基因质粒和生理盐水。4周后观察基因表达和促血管新生效应。 结果与单纯基因治疗组相比超声激活携基因微泡组荧光强度和bFGF mRNA表达水平显著提高（P〈0.05）,且血管新生更明显（P〈0.05）。 结论超声激活携基因微泡可明显提高bFGF基因在鼠活体心肌的表达,促进血管新生。Objective To investigate expression of foreign gene in ischemic myocardium by ultrasound-mediated microbubble destruction,and to explore the feasibility of therapeutic angiogenesis in myocardial infarction.Methods The intramyocardial injection was chosen for gene delivery.Mixtures of basic fibroblast growth factor gene （bFGF） plasmids and microbubbles were injected in ultrasound-mediated microbubbles group with simultaneous ultrasound irradiation,bFGF gene plasmids were infused in gene group,and saline were infused in control group.After 4 weeks,the expression of gene and its proangiogenesis effect were observed.Results The red fluorescence intensity and the expression of bFGF at mRNA level were higher in the U group than in P group（P〈0.05）. After delivering by ultrasound mediated microbubble cavitation,the microvessel density counting was significant enhanced than the P group（P〈0.05）.Conclusions Ultrasound-mediated microbubble destruction can effectively enhance the expression of gene such as bFGF into the infarcted myocardium of rats and stimulate angiogenesis.

关 键 词：微泡 超声 心肌梗死 血管新生 

分 类 号：R644[医药卫生—外科学] R776.1[医药卫生—临床医学]