植物游离小孢子及其培养所获得的组织在遗传转化中的应用  

Applications of Plant Isolated Microspores and Microspore-derived Tissues in Genetic Transformation

作　　者：常凯军[1] 谭德冠[1] 李定琴[1] 郑鹏[1] 张家明[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点开放实验室,海口571101

出　　处：《中国农学通报》2009年第15期16-21,共6页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金资助(30260062;30660152);中国热带农业科学院中央级公益性科研院所基本科研业务费专项资金资助(IT-BBDZ0711)

摘　　要：植物游离小孢子及其培养所获得的组织是植物基因工程中优良的受体,它们具有单倍性和较高胚胎发生能力的特性。因此,以它们为受体构建遗传转化体系可以快速获得纯合的转基因植株。通过综述植物游离小孢子及其培养所获得的胚状体、愈伤组织作为转化受体在根癌农杆菌介导法、基因枪轰击法、激光微孔穿刺法、显微注射法、电激法、PEG介导法等转化技术中的应用研究进展,对目前游离小孢子作为转化受体存在的问题及发展前景进行了探讨。Based on their characteristics of haploid and high embryogenetic spores and microspore-derived tissues are the good acceptors in plant genetic capacity, plant isolated microengineering. Therefore, the homozygous transgenic plants could be obtained more quickly using them as acceptors to construct genetic transformation system. The applications of plant isolated microspores, microspore-derived embryos and microspore-derived calli as aeceptors in several transformational technology including Agrobacterium co-cultivation, particle bombardment, laser microbeam puncture, microinjection, electroporation, polyethylene glycol （PEG）-mediated were reviewed. Furthermore, problems and prospects of microspores as transformation acceptors were discussed in this review.

关 键 词：游离小孢子 游离小孢子培养来源的胚状体 遗传转化 根癌农杆菌介导法 基因枪轰击法 

分 类 号：S335.4[农业科学—作物遗传育种]