拟南芥基因组NBS-LRR类基因家族的生物信息学分析  

Bioinformatic Analysis of the NBS-LRR Gene Family in Arabidopsis thaliana

作　　者：王岩[1] 李兆阳[1] 唐心龙[1] 卢姗[1] 许鹏[1] 张静[1] 方奎[1] 席景会[1] 

机构地区：[1]吉林大学植物科学学院,长春130062

出　　处：《中国农学通报》2009年第15期40-45,共6页Chinese Agricultural Science Bulletin

摘　　要：【研究目的】对拟南芥(Arabidopsis thaliana)中含有核苷酸结合位点(Nucleotide binding site,NBS)和富含亮氨酸的重复序列(Leucine-richrepeat,LRR)类基因进行了分析。【方法】利用TAIR、Pfam网站,数据库和Chromosome Map Tool、MEGA3.0、ClustalX软件,分析确定NBS-LRR基因及其类型、染色体物理分布、系统发育学关系。【结果】在拟南芥全基因组中共有204个NBS-LRR类基因,大多位于1号和5号染色体上,其中71.6%的NBS-LRR类基因分布在基因簇内。对NBS-LRR类基因家族进行了氨基酸多序列比对和系统进化树分析,NBS-LRR类基因家族可分为四个亚家族。另外,NBS-LRR基因在进化过程中存在较多的复制现象。[OBJECTIVE]A full set of disease resistance candidate genes encoding NBS-LRR in a complete genome ofArabidopsis thaliana were analyzed. [METHOD] Structural diversities, physical positions, and phylogenetic relationship were studied by TAIR and Pfam web, database, Chromosome Map Tool, MEGA 3.0 and ClustalX software. [RESULTS] 204 genes containing NBS domain were found in genome and most of those were located in 1 and 5 chromosome. The NBS-LRR family genes on different chromosomes exist in the form of monogenic gene or gene clusters, and 71.6% of the NBS-LRR genes were located in gene clusters. That showed there are much copy phenomenons in the evolution of disease resistance genes ofArabidopsis thaliana.

关 键 词：拟南芥 生物信息学 基因家族 NBS—LRR 

分 类 号：S432.23[农业科学—植物病理学]