宁夏回族和汉族老年急性冠状动脉综合征患者凝血因子Ⅶ基因多态性的分布特征  

The characteristics of distribution of F Ⅶ gene polymorphism in Ningxia Hui and Han elderly with acute coronary syndrome

作　　者：黄晖[1] 贾绍斌[1] 沙勇[1] 陈树兰[1] 马爱群[2] 陈中青[1] 张金莉[1] 何琳[1] 

机构地区：[1]宁夏医科大学附属医院心脏中心,银川750004 [2]西安交通大学第一附属医院心内科

出　　处：《中华老年心脑血管病杂志》2009年第8期573-577,共5页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：宁夏回族自治区教育厅基金(J200555)

摘　　要：目的探讨血浆凝血因子Ⅶ(FⅦ)基因多态性在宁夏回、汉族老年急性冠状动脉综合征(ACS)患者中的分布特征。方法选择冠心病患者664例分为回族冠心病组(336例)和汉族冠心痛组(328例),其中回族ACS患者194例,汉族ACS患者175例;同期选择健康体检者750例分为回族对照组(400倒)和汉族对照组(350例),采用候选基因及病例对照研究的方法,以聚合酶链反应-限制性片段长度多态性技术,行FⅦ基因的R353Q、-3230/10hp和HVR4基因多态性分析,同时采用重组可溶性组织因子法测定血浆FⅦa水平。结果回、汉族冠心痛组患者的血浆FⅦa水平均显著高于同族对照组(P<0.01)。R353Q基因型及等位基因频率分布在回族冠心痛组与回族对照组差异有统计学意义(P<0.01),-323 0/10 bp基因型及等位基因频率分布在汉族冠心痛组与汉族对照组差异有统计学意义(P<0.01)。回族ACS患者Q/Q+R/Q基因型频率高于回族非ACS患者,差异有统计学意义(P<0.01)。结论宁夏回、汉族人群中存在FⅦ基因多态性;Q等位基因可能是回族人群ACS发生的遗传保护因子,FⅦa水平受R353Q基因多态性影响。Objective To investigate the characteristics of distribution of coagulation factor （FⅦ） gene polymorphism in Ningxia Hui and Han patients with ACS. Methods 664 patients with CHD in Ningxia proven by angiography（Hui 336, Han 328） were compared respectively with 400 Hui and 350 Han healthy blood donors serving as the control group. Their plasma levels of FⅦ was detected using recombinant soluble tissue factor method. The FⅦ gene R353Q,- 323 0/10 bp and HVR4 genotypes were identified by polymerase chain reaction amplified genomic DNA method. Results Plasma FⅦa levels were significantly higher in patients with CHD than in control group. The distribution of genotypes and allele frequencies of R353Q in the Hui patient and control groups displayed significant difference, and there was also difference between the subgroups of ACS and non-ACS （P 〈 0.05）. The difference of the -323 0/10 bp existed between patients with CHD and controls in the Han. FⅦ a level was significantly higher in RR genotype than in Q allele carriers in the Hui. Conclusion The FⅦ gene polymorphism is present in the Hui and Han population in Ningxia. The Q allele may be a protective factor against CHD in the Hui, and plasma FⅦa level may be influenced by the R353Q polymorphism.

关 键 词：因子Ⅶ 冠状动脉疾病 多态性 限制性片段长度 基因频率 回族 汉族 

分 类 号：R541.4[医药卫生—心血管疾病]