地鳖纤溶活性蛋白对H22荷瘤小鼠的抑瘤作用  

The inhibitory effects and immunoregulatory activity of the fibrinolytic protein from Eupolyphaga sinensis Walker (EFP) in vivo on line H22 in mice

作　　者：韩雅莉[1] 郭桅[1] 李兴暖[1] 谭竹钧[1] 

机构地区：[1]广东工业大学轻工化工学院生物工程系,广东广州510090

出　　处：《中国药理学通报》2009年第7期900-903,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30772739)

摘　　要：目的探讨地鳖纤溶活性蛋白(fibrinolytic protein from Eupolyphaga sinensis Walker,EFP)对H22荷瘤小鼠的抑瘤功效。方法采用水提取法对地鳖进行成分分离;皮下接种肿瘤细胞建立荷瘤小鼠动物模型;灌胃和皮下注射法观察EFP的抑瘤作用;ELISA法检测血清抗H22抗体水平;记录各组体重变化、脾脏和胸腺等免疫器官的器官系数,检测肝脏丙二醛(MDA)水平、肝脏超氧化物歧化酶(SOD)活性、肝脏谷丙转氨酶(GPT)和谷草转氨酶(GOT)以及血清谷丙转氨酶(GPT)活力等生理生化指标。结果EFP具有明显的抑瘤活性。经EFP灌胃和皮下注射后的荷瘤小鼠,与对照组荷瘤小鼠相比其胸腺指数、脾指数明显增加;抗H22抗体水平以及MDA、SOD、GPT、GOT活性明显增加;肿瘤生长缓慢。结论EFP是地鳖抗肿瘤作用的主要有效成分,EFP在动物体内具有抗肿瘤作用,能通过增强荷瘤小鼠参与免疫抗体以及相关酶的活性而发挥抗肿瘤作用。Aim To explore the inhibitory effects and immunoregulatory activity of the extract from Eupolyphaga sinensis Walker （EFP） on the growth of liver cancer H22 in mice. Methods The chemical composition of extract from Eupolyphaga sinensis Walker（EFP） was separated with water extraction methods. The tumor bearing mouse model was constructed by injecting tumor cells subcutaneously. The EFP was given to the mouse by oral and hypodermic injection to observe its antitumor activity and immunoregulatory activity in vivo. The antibody level of anti-H22 in serum was measured with ELISA methods. The weight, tumor weight, spleen index, liver index were noted, and series of enzymes,such as, MDA, SOD, GPT and GOT activity of mouse serum and liver were measured. Results The extracts from Eupolyphaga sinensis Walker markedly inhibited the proliferation of tumor H22. The EFP constit- uent purified might be the main antitumor active part of Eupolyphaga sinensis Walker as shown by antitumor test in vitro. After treatment with EFP, the general condition of the test mice was much better than that of control mice, with spleen index, liver index increasing more obviously, antibody level of anti-H22, and MDA, SOD, GPT and GOT activity increasing more, and tumor growing more slowly. Conclusion EFP may be the main antitumor active part of Eupolyphaga sinensis Walker. EFP also has antitumor effect in vivo. EFP can exert antitumor effect in vivo by enhancing antibody level of anti-H22 and the series enzymes to be related to immunity activity.

关 键 词：地鳖虫 H22 ELISA 抑瘤作用 纤溶活性蛋白质 纯化 

分 类 号：R282.74[医药卫生—中药学] R329.24[医药卫生—中医学]