多廿醇对人单核细胞低密度脂蛋白受体活性的影响  

Effect of policosanol on activity of LDL-R

作　　者：何凤英[1] 庞伟毅[1] 陆继培[1] 韦小敏[1] 

机构地区：[1]广西医科大学公共卫生学院卫生毒理学教研室,广西南宁530021

出　　处：《中国药理学通报》2009年第7期946-948,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30360091)

摘　　要：目的研究多廿醇对低密度脂蛋白受体(low density lipoprotein receptor,LDL-R)活性的影响,探讨多廿醇降脂作用的机制。方法体外试验采用常规细胞培养方法,观察多廿醇对人单个核细胞LDL-R的直接作用。体内试验采用自身和组间两种对照设计,观察多廿醇对高胆固醇血症人群LDL-R活性的影响。LDL-R活性检测选用荧光标记配体法。结果体外试验表明多廿醇在5～20mg·L-1范围内可以增强人单个核细胞LDL-R的活性,并且具有明显的量-效关系。体内试验中高胆固醇患者服用多廿醇20mg·d-14wk后,外周血单个核细胞LDL-R活性升高95%。结论多廿醇能通过增加LDL-R的活性而起降血脂作用,多廿醇降血脂是多途径的。Aim To identify the effect of policosanol on LDL-R activity. Methods In vitro cell culture experiments conventional methods were used to observe the direct effect of policosanol on mononuclear cells LDL-R. In vivo experiments self-control and negative-control group design methods were used to observe the effect of policosanol on LDL-R activity in the patients with hy- percholesterolemia. LDL-R activity was analysed by flu- orescence flow cytometry and labeled by the fluorescent reagent DiI. Results Policosanol 5 - 20 mg · L^-1 obviously activated the activity of LDL-R in human mono- nuclear cells, policosanol levels and the activity of LDL-R in human mononuclear cells showed significantly dose-effect relationship in vitro study. These tendency could also be seen in the patients with hypercholes- terolemia after policosanol treatment for four weeks in vivo study. As the increasing of policosanol dose, the activity of LDL-R in human mononuclear cells remarkably increased in the patients with hypercholesterolemia Conclusions Policosanol up-regulates the activity of the LDL-R in the mononuclear ceils and reduce cholesterol level in the body. Policosanol reduce lipids through multiple ways including LDL-R.

关 键 词：多廿醇 低密度脂蛋白受体 人单个核细胞 荧光标记 高胆固醇血症 临床试验 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R392.11[医药卫生—基础医学]