VEGF转基因神经干细胞移植对大鼠急性放射性脑损伤后脑组织中NSE表达的影响  

Effect of neural stem cells transfected with VEGF on expression of NSE in rat brain injury irradiated by radioaction

作　　者：肖颂华[1] 段朝晖[2] 沈庆煜[1] 邢诒刚[1] 彭英[1] 陶恩祥[1] 刘军[1] 

机构地区：[1]中山大学附属第二医院神经科,广东广州510120 [2]中山大学附属第二医院检验科,广东广州510120

出　　处：《中国病理生理杂志》2009年第8期1559-1563,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30870750);广东省自然基金博士启动资助项目(No.8451008901000672);广东省医学科研基金资助项目(No.B2008044);中山大学校青年教师基金资助项目(No.3177915)

摘　　要：目的:探讨血管内皮生长因子(VEGF)转染的神经干细胞(NSC)对放射性脑损伤的影响。方法:将Sprague-Dawley(SD)大鼠随机分为对照组、放射性脑损伤组、NSC和转染VEGF的NSC治疗组,放射性脑病模型成功制备后1周脑内移植转染VEGF的NSC,移植后1周、2周、3周、4周和6周末取其脑组织,用免疫组织化学法测定神经元特异性烯醇化酶(NSE)阳性细胞数,Western blotting法检测NSE蛋白表达情况。结果:与对照组相比,放射性脑损伤组大鼠脑组织中NSE阳性细胞数显著下降(P<0.05);与放射性脑损伤组相比,NSC治疗组和转染VEGF的NSC治疗组在移植3周后NSE阳性细胞数明显增加(P<0.05),与NSC治疗组比较,在移植6周末转染VEGF的NSC治疗组NSE的阳性细胞数增加更明显(P<0.05)。与放射性脑损伤组比,NSC治疗组和转染VEGF的NSC治疗组在移植3周末NSE蛋白表达量明显增加(P<0.05),与NSC治疗组比较,在移植6周末转染VEGF的NSC组NSE蛋白表达量增加更明显(P<0.05)。结论:转染VEGF的NSC移植可提高放射性脑损伤组织中NSE的含量。AIM ： To explore the effect of neural stem cells （NSC） transfected with vascular endothelial growth factor （VEGF） on brain injury irradiated by radioaction. METHODS： Mature Sprague -Dawley （SD） rats were randomly divided into 4 groups： control group, radiation brain injury group, NSC therapeutic group, NSC transfected with VEGF （NSC/VEGF） therapeutic group. NSC transfected with VEGF was transplanted into the rat brain of therapeutic group after preparation of radiation encephalopathy model for 1 week. The brain tissue was taken at week 1,2, 3, 4 and 6 after trans- plantation. Immunohistochemical method was used to assay the count of neuro - specific enolase （NSE） stained cells in brain tissue. Westem blotting was used to detect the expression of NSE protein. RESULTS： Compared to control group, the count of NSE stained cells in brain of radiation brain injury group was decreased significantly （P 〈0. 05）. Compared to that in radiation brain injury group, the count of NSE stained cells in brains was enhanced in NSC therapeutic group and NSC/VEGF therapeutic group since 3 weeks after transplantation （P 〈 0. 05 ）. Compared to that in NSC therapeutic group, the count of NSE stained cells in NSC/VEGF therapeutic group was more enhanced since 6 weeks after transplantation （P 〈 0. 05）. Compared to that in radiation brain injury group, the expression of NSE protein was enhanced in NSC group and NSC/VEGF group since 3 weeks after transplantation （P 〈 0. 05）. Compared to that in NSC group, the expression of NSE protein in NSC/VEGF group was more enhanced since 6 weeks after transplantation （P 〈 0. 05 ）. CONCLUSION： Trans-plantation of NSC transfected with VEGF may increase the content of NSE in rat brain of radiation encephalopathy model.

关 键 词：血管内皮生长因子 神经元特异性烯醇化酶 神经干细胞 辐射脑损伤 

分 类 号：R818[医药卫生—放射医学]