十二烷基苯磺酸钠存在下共振光散射法测定亚甲蓝  

Determination of Methylene Blue by a Resonance Light Scattering Technique with Dodecyibenzene Sulfonic Acid Sodiumsalt

作　　者：刘飒[1] 刘绍璞[1] 谢锂纱[2] 胡小莉[1] 宋彦琪[1] 

机构地区：[1]西南大学发光与实时分析教育部重点实验室,化学化工学院,重庆400715 [2]四川出入境检验检疫局,成都

出　　处：《应用化学》2009年第8期966-970,共5页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金(20475045);发光与实时分析重庆市重点实验室(西南大学)(CSTC,2006CA8006);重庆市科委自然科学基金计划(CSTC,2007BB1359)资助项目

摘　　要：在0.05 mol/L(pH=1.3)的HCl介质中,十二烷基苯磺酸钠(SDBS)与亚甲蓝(MB)通过静电引力和疏水作用力形成2∶1的离子缔合物,导致溶液共振瑞利散射(RRS)、二级散射(SOS)和倍频散射(FDS)急剧增强,光谱最大散射强度分别位于310、648和341 nm,并在一定范围内与MB的浓度成正比,对于MB的检出限(3σ)分别为1.2×10-9g/mL(RRS法)、1.4×10-9g/mL(SOS法)和1.7×10-9g/mL(FDS法)。据此建立了光散射法测定痕量亚甲蓝的新方法。用于人血清样品中亚甲蓝含量的检测,回收率在94.4%～103.7%之间。实验优化了反应条件,考察了共存物质的影响,讨论了反应机理和散射光谱产生及增强的原因。In 0.05 mol/L（pH=1.3） HCl,SDBS combined with methylene blue（MB） to form an ion-association complex with a composition ratio of 2∶1 by electrostatic and hydrophobic interactions.As a result,the intensities of RRS,FDS,and SOS spectra were enhanced greatly.The maximum wavelengths of RRS,SOS and FDS were located at 310 nm,648 nm and 341 nm respectively,and the scattering intensities were propo-rtional to the concentration of MB.The detection of MB using these three compounds has very high sensitivities,and the detection limits were 1.2×10^-9 g/mL for RRS,1.4×10^-9 g/mL for SOS and 1.7×10^-9 g/mL for FDS,respectively.At the same time,the methods have better selectivity.Based on the above results,a new spectral method for the determination of trace amounts of MB has been developed.It can be applied to the determination of MB in human serum with a satisfactory result,and the recoveries are 94.4%103.7%.In this work,the optimum conditions of the reaction and the effects of coexisting substances were investigated.In addition,the reaction mechanism and the reasons of enhancement of resonance light scattering were discussed.

关 键 词：十二烷基苯磺酸钠 共振瑞利散射 二级散射 倍频散射 亚甲蓝测定 

分 类 号：O657.3[理学—分析化学]