两种电生理方法研究心梗大鼠转SERCA2a基因对心肌电活动的影响  

The Comparison of two methods to study for alternation of electrophysiology in rat hearts after myocardial infarction by overexpression of the SERCA2a

作　　者：马晓洁[1] 范平[1] 张春[1] 刘政疆[1] 侯月梅[1] 

机构地区：[1]新疆医科大学第一附属医院心律失常实验室,新疆乌鲁木齐830011

出　　处：《新疆医科大学学报》2009年第4期408-411,共4页Journal of Xinjiang Medical University

基　　金：国家自然科学基金项目(编号:30560050)

摘　　要：目的:采用微电极阵列技术(MEA)与局部电位探查法观察大鼠心梗周围区转SERCA2a基因后心肌电生理的变化,并对这两种方法进行比较。方法:50只成年雄性SD大鼠随机平均分为盐水组和SERCA2a基因组。结扎左冠状动脉前降支建立大鼠心梗模型。术中全程行心电监测,术后24h内大鼠存活30只,两周后盐水组存活10只;SERCA2a组存活15只。基因转导采用梗死心肌周围组织局部多点直接注射法,分别用组织微电极阵列技术(MEA)与双极电位标测技术观察干预2周后,心肌组织间场电位时程(FPd)与局部电位时程的变化。结果:造模成活率为60%,大鼠结扎冠脉后30min可见ST段抬高,Q波形成。2周后MEA显示:两组梗塞周围区场电位时程较梗塞对侧均有所缩短,但转SERCA2a基因组场电位离散度显著小于盐水组,双极电位标测结果显示转SERCA2a基因组梗塞周围区局部电位时程长于对侧区,而盐水组梗塞周围区局部电位时程短于对侧区,但转基因组场电位离散度显著小于盐水组,差异均有统计学意义(P<0.05)。结论:两种方法均证实转SERCA2a基因可以缩短心肌梗塞后心肌电位时程离散度。因此提示转SERCA2a基因有预防心梗后心律失常的作用。MEA技术在观察心梗周围组织电活动一致性更为理想。Objective： Using two electrophisiological methods to study the therapy effect of SERCA2a genetransfer on arrhythmia after acute myocardial infarction rats in 2 weeks. To compare the effect of the two methods and analyze the possible mechanism. Methods： Fifty rats were divided into three groups： control group （n =30）, SERCA2a group （n = 15）, and saline group （n = 10）. Acute myocardial infarction rats were obtained by ligating left anterior descending coronary artery. 0.9 % sodium chloride solution and recombinant adenovirus carrying SERCA2a gene were respectively delived to the heart of AMI rats in two groups by directed injection around the ischemie area. Electrocardiogrph were tested immediately after surgery. Two weeks after gene transfer, field potentail and local potential were detected respectively by mi croeleetrode arrays and dipolar electric potential mapping. Results： Two weeks after gene transfer, survivle rate of the model rats was 60%. MEA and dipolar electric potential mapping showed that in two weeks, the dispersion of the field potential duration and local potential duration was longer in saline group than it in SERCA2a group （P 〈0.05）. Conclusions： Adenoviral gene transfer of SERCA2a can reduce the incidence of arrhythmia after acute myocardial infarction. MEA and dipolar electric potential mapping are idealmethods to study the concordence of the myocardia in different part of whole heart.

关 键 词：双极电位标测 微电极阵列 急性心肌梗塞 转SERCA2a基因 

分 类 号：R-332[医药卫生] R54