银杏叶提取物血清药理学方法的建立及验证  

Establishment and validation of serum pharmacology method for Ginkgo biloba extract

作　　者：魏雅芹[1] 汤道权[1] 高媛媛[1] 印晓星[1] 陈永刚[1] 韩琳[1] 

机构地区：[1]徐州医学院药学院,徐州221004

出　　处：《中药药理与临床》2009年第3期74-77,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：江苏高校自然科学基金项目资助;项目编号:07KJD360225

摘　　要：目的:建立并验证银杏叶提取物(GBE)血清药理学研究方法。方法:MTT法确定体系中血清添加量;HPLC法结合药效法确定大鼠给药方案及采血时间;以T-SOD、MDA为指标测定该含药血清的抗氧化能力。结果:大鼠按人临床用药等效剂量的5倍,每日两次连续4天灌胃给予GBE,于末次给药后2h采血时血清中GBE各有效成分的含量最高;体系中加入20%该条件下制备的含药血清对大鼠肾小球系膜细胞(MC)正常增殖没有影响;采用该方法制备的GBE含药血清具有明显的抗氧化能力。结论:本实验条件下制备的GBE含药血清具有明显的抗氧化能力,该血清药理学方法能用于GBE含药血清药效及机制研究。To establish and validated a serum pharmacology method for Ginkgo biloba extract （GBE）. Methods： MTT assay was used to determine the suitable concentration of blank serum in culture system. HPLC method was use to determine the optimum dosage of administration and time of hemospasia. T-SOD and MDA was selected to validate the antioxidant effect of serum containing GBE. Results： Rats were oral administration of GBE by 5 times dose of adults twice a day, keeping on 4 days. Hemospasia after 2 hours of the last administration was the best preparative method of the serum containing drug. Adding 20% of this serum to culture system has no influence on multiplication of mesangial cells （MC）. This serum containing GBE has obvious antioxidant effect on MC. Conclusion： Serum containing GBE prepared by suitable method possesses obvious antioxidant effect and this serum pharmacology method can be applied to evaluate the pharmacological effect and mechanism of GBE.

关 键 词：银杏叶提取物 血清药理学 抗氧化 HPLC 

分 类 号：R285[医药卫生—中药学] R285.5[医药卫生—中医学]