Determination and Analysis of Mitochondrial ND2 Gene Sequence of Anas platyrhynchos  

绿头鸭线粒体ND2基因全序列测定及其研究(英文)

作　　者：涂剑锋[1] 司方方[1] 邢秀梅[1] 杨福合[1] 

机构地区：[1]中国农业科学特产研究所,吉林吉林132109

出　　处：《Agricultural Science & Technology》2009年第3期46-49,共4页农业科学与技术（英文版）

基　　金：Supported by National Key Technology R&D program(2006BAD06B06);National Infrastructure of Natural Resources for Science and Technology(2004DKA30460)~~

摘　　要：[Objective] The study was to analyze the phylogenesis of Anas platyrhynchos. [Method] Complete sequence of mitochondrial ND2 gene of 4 Anas platyrhynchos was determined by direct DNA sequencing based on PCR products. Combined with ND2 gene sequences of the Anas Linnaeus accessed in GenBank, phylogenetic tree was constructed by Neighbor-joining and maximum parsimony methods. [Result] The ND2 gene sequences of 4 Anas platyrhynchos were identical(1 041 bp in length; the nucleotide contents of A, G, T, and C were 28.91%, 13.35%, 20.75% and 36.98% respectively; A+T content approximated to that of C+G). Sequences of ND2 gene of mallard were same as spotbill duck, and had high homology with others. The phylogenetic trees indicated mallard and spotbilled duck were close in genetic relationship, both shared a haplotype; then Philippine duck, green-winged teal and northern pintail fell into branch ''A". [Conclusion] The domestic duck may be domesticated from mallard and spotbilled duck.[Objective] The study was to analyze the phylogenesis of Anas platyrhynchos. [Method] Complete sequence of mitochondrial ND2 gene of 4 Anas platyrhynchos was determined by direct DNA sequencing based on PCR products. Combined with ND2 gene sequences of the Anas Linnaeus accessed in GenBank, phylogenetic tree was constructed by Neighbor-joining and maximum parsimony methods. [Result] The ND2 gene sequences of 4 Anas platyrhynchos were identical（1 041 bp in length; the nucleotide contents of A, G, T, and C were 28.91%, 13.35%, 20.75% and 36.98% respectively; A＋T content approximated to that of C＋G）. Sequences of ND2 gene of mallard were same as spotbill duck, and had high homology with others. The phylogenetic trees indicated mallard and spotbilled duck were close in genetic relationship, both shared a haplotype; then Philippine duck, green-winged teal and northern pintail fell into branch ＇＇A＂. [Conclusion] The domestic duck may be domesticated from mallard and spotbilled duck.

关 键 词：Anas platyrhynchos ND2 gene sequence analysis Phylogenetic tree 

分 类 号：S865[农业科学—野生动物驯养]