蚋类唾腺多线染色体制备方法的改进  

Improvement for the preparation of the polytene chromosomes of blackflies

作　　者：黄丽[1] 张春林[1] 陈汉彬[1] 

机构地区：[1]贵阳医学院生物学教研室,贵阳550004

出　　处：《中国媒介生物学及控制杂志》2009年第4期281-283,共3页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金(39460073)

摘　　要：目的对传统蚋类唾腺多线染色体制备方法进行改进,以获得良好的制片效果。方法选用经Carnoy’s液固定的蚋成熟幼虫进行解剖、剥离唾腺,对传统蚋类唾腺多线染色体制备方法进行改进,包括改良染色液,使用冲洗液、背景净化液等新制剂和新步骤并得到清晰的蚋唾腺染色体图形。结果用改进方法制备的蚋类唾腺多线染色体标本带型清晰,伸展良好。结论改进的蚋类唾腺多线染色体制备方法可获得较满意效果,便于观察染色体形态和研究染色体结构变异等。Objective To improve the traditional preparation of the polytene chromosomes of blackflies and observe chromosomes abstracted. Methods Mature larvae of blackflies were selected, dissected and shelled out salivary glands. The traditional method was improved, and the clear polytene chromosomes slices were prepared after the use of new staining fluid, flush fluid and cleaning fluid. Results The improved method made chromosomes clearly and wholly in microscope. Conclusion The improved technique succeeded in the preparation of chromosome specimens of blackfly salivary gland, which was convenient for the observation of chromosomes shape and chromosomes variation in the laboratory.

关 键 词：蚋科 唾腺 多线染色体 制备方法 

分 类 号：Q969.44[生物学—昆虫学]