衣原体等感染因子与眼附属器黏膜相关淋巴组织淋巴瘤相关性的研究  

Relationship between primary ocular adnexal mucosa.associated lymphoid tissue lymphoma and eye infection

作　　者：张丹丹[1] 刘红刚[2] 李海燕[3] 高子芬[4] 周小鸽[5] 金哈斯[6] 董丽娜[1] 张婧[1] 宫丽平[1] 

机构地区：[1]首都医科大学病理学教研室,北京100069 [2]首都医科大学附属北京同仁医院 [3]山西省眼科医院病理科 [4]北京大学医学部病理学系 [5]首都医科大学附属北京友谊医院 [6]北京武警总医院血液科

出　　处：《中华病理学杂志》2009年第8期513-518,共6页Chinese Journal of Pathology

基　　金：北京市教育委员会科技发展计划项目（KM200810025017）;首都医科大学基础-临床合作基金（2006JL02）;首都医科大学科研基金（自然科学）（2006ZR05）

摘　　要：目的探讨眼部慢性炎症相关病原微生物：鹦鹉衣原体（Cps）、沙眼衣原体（Ctr）、肺炎衣原体（Cpn）、单纯疱疹病毒（HSV）1和2及腺病毒（ADV）8和19与眼附属器黏膜相关淋巴组织结外边缘区淋巴瘤（MALT淋巴瘤）之间的关系，为探寻眼附属器MALT淋巴瘤的病因及发病机制提供线索。方法收集原发性眼附属器淋巴增生性病变石蜡包埋组织标本68例，其中MALT淋巴瘤38例，非MALT淋巴瘤3例（弥漫性大B细胞淋巴瘤2例，滤泡性淋巴瘤1例），慢性炎症27例。提取以上组织的DNA，并以PCR扩增来控制质量，选取质量合格的DNA，应用touchdown enzyme time—release PCR（TETR—PCR）法，分别检测Cps、Ctr、Cpn、HSV1和2及ADV8和19的感染状况。结果所有样本的DNA长度均达100bp，适用于TETR—PCR法检测感染源。其中，Cps、Ctr及ADV19在38例MALT淋巴瘤、3例非MALT淋巴瘤及27例慢性炎症中的感染率均为0；Cpn在MALT淋巴瘤中有2例为阳性（5．3％，2／38），但在非MALT淋巴瘤和慢性炎症中均为阴性（P〉0．05，Fisher确切概率法）；HSV1和2及ADV8在慢性炎症中均有1例阳性（1／27），但在MALT淋巴瘤及非MALT淋巴瘤中均为阴性（P〉0．05，Fisher确切概率法）。结论中国眼附属器MALT淋巴瘤的发生与Cps、Ctr、Cpn、HSV1和2及ADV8和19的感染无明显相关性。Objective To study the role of pathogenic microorganisms commonly associated with chronic eye disease, including Chlamydia psittaci, Chlamydia trachomatis, Chlamydia pneumoniae, herpes simplex virus （HSV） type 1 and type 2, and adenovirus type 8 and type 19, in the development of primary ocular adnexal mucosa-associated lymphoid tissue （MALT） lymphoma in Chinese patients. Methods Sixty-eight archival cases of primary ocular adnexal lymphoproliferative lesions, including 38 cases of MALT lymphoma, 3 cases of non-MALT lymphoma and 27 cases of chronic inflammation, were enrolled into the study. DNA was extracted from the paraffin-embedded tissue samples. The presence of DNA of C. psittaci, C. trachomatis, C. pneumoniae, HSV type 1, HSV type 2, adenovirus type 8 and adenovirus type 19 were analyzed by multiplex touchdown enzyme time-release polymerase chain reaction （TETR-PCR）. Results All of the specimens yielded PCR products of over 100 base pairs and were thus suitable for TETR-PCR screening of infectious agents. The prevalence of DNA of C. psittaci, C. trachomatis and adenovirus type 19 were 0 in MALT lymphoma, non-MALT lymphoma and chronic inflammation. There were 2 cases positive for C. pneumoniae DNA, amongst the 38 cases of MALT lymphoma studied （5.3%, 2/38）. HSV type 1, HSV type 2 and adenovirus type 8 DNA was found in each of the 3 patients with chronic inflammation. Conclusion The study indicates that C. psittaci, C. trachomatis, C. pneumoniae, HSV type 1, HSV type 2, adenovirus type 8 and adenovirus type 19 probably play little role in the pathogenesis of ocular adnexal MALT lymphoma in Chinese patients.

关 键 词：眼肿瘤 淋巴瘤 黏膜相关淋巴样组织 衣原体 鹦鹉 聚合酶链反应 

分 类 号：R686[医药卫生—骨科学]