猪胞内劳森菌16SrRNA基因的克隆及序列分析  

Cloning and sequencing analysis of 16SrRNA gene of Lawsonia intracellularis

作　　者：谢丽华[1,2] 张宁[1] 何苹萍[1] 李茂宁[1] 尹业师[1] 韦英益[1] 肖爱欢[1] 黄伟坚[1] 

机构地区：[1]广西大学动物科学技术学院,广西南宁530005 [2]广西梧州市动物疫病预防控制中心,广西梧州543002

出　　处：《中国兽医学报》2009年第8期968-972,共5页Chinese Journal of Veterinary Science

基　　金：广西科学研究与技术开发计划项目基金资助项目(桂科教0630006-6B)

摘　　要：胞内劳森菌（Lawsonia intracellularis,LI）是猪增生性肠炎的病原,本试验设计3条引物,采用半巢式PCR,从猪的回肠粘膜中扩增出长为1 457 bp的胞内劳森氏菌16SrRNA基因,并进行序列比较分析。结果表明,与不同动物源性（鼠、仓鼠、鹿、马、鸵鸟等）的增生性肠炎胞内菌核苷酸同源性在98.4%-100%之间,与脱硫弧菌同源性达到88.6%,与一般弯曲杆菌和螺旋体同源性较低（71.8%-73.9%）。Lazvsonia intracellularis （LI）is a newly discovered etiological agent,which causes porcine proliferative enteropathy that cuts down feed conversion rate and threatens hog industry seriously. However,there is a lack of reports on sequencing analysis of this etiological agent gene in domestic. In this study, we designed 3 primers to amplificate LI 16SrRNA gene consisted of 1 457 bp,through semi-nested PCR,and then made sequence analysis. The result showed that the isolate from this study had 98.4% to 100% nucleotide sequence homology with the isolates from other species of anmals （such as mouse,hamster,deer,horse and ostrich etc. ） ,88.6% homology with D. desulfuricans,and low homology （from 71.8% to 73.9%） with Campylobacter species and Spirochaeta. It is a first report on the gene clone and sequence analysis of 16SrRNA gene of Lawsonia intracellularis in domestic,and gives a reference basis to the molecular diagnostic methods and classification for this etiological agent.

关 键 词：胞内劳森氏菌 16SrRNA 序列分析 

分 类 号：S852.61[农业科学—基础兽医学]