PRV基因缺失株在体外细胞中增殖的超微结构  

Ultrastructure of the PRV gene-deleted strains in cell proliferation in vitro

作　　者：徐志文[1] 郭万柱[1] 朱玲[1] 徐凯[1] 王印[1] 王小玉[1] 

机构地区：[1]四川农业大学动物生物技术中心,四川雅安625014

出　　处：《中国兽医学报》2009年第8期1008-1012,共5页Chinese Journal of Veterinary Science

基　　金：国家"十一五"科技支撑计划资助项目(2006BAD06A18-3);四川省教育厅重点实验室专项(07ZZ027)

摘　　要：用伪狂犬病病毒(Pseudorabies virus,PRV)Fa株和本实验室构建的系列PRV基因缺失株(PRV-TK-、PRV-gI\gE-、PRV-TK\gI\gE-株)感染不同的细胞系(IBRS-2、Marc145、ST、Vero、MDBK细胞),对感染细胞的形态学观察发现,受试各PRV毒株在以上细胞均能增殖并引起细胞病变,但对不同的细胞却表现差异,其中在ST细胞上增殖滴度最低,在MDBK上最高;同PRV Fa株相比,PRV-gI\gE-和PRV-TK\gI\gE-株的增殖力有所降低,而PRV-TK-则变化不明显;对细胞感染后的超微结构分析发现,基因缺失对该毒株在细胞上的吸附和穿入过程没有影响,但与Fa株相比,主要表现为增殖速度的减缓和病毒粒子数量的减少。Different kinds of cells（IBRS-2, Marc145, ST, Vero, MDBK） were inoculated wifh the PRV Fa strain and the PRV gene-deleted strains constructed by Animal Biotechnology Center of Sichuan Agricultural University. According to the morphology observation of the infected cells, we found that the PRV strains could proliferate in these cells and caused CPE. However,different cells performed a difference. The PRV strains were more sensitive in MDBK, but less sensitive in ST. The proliferative ability of the PRV-gI／gE^- , PRV-TK ／gI／gEa^- strains were lower than the PRV Fa strain,however the PRV-TK^- strain show a little difference. According to the ultrastructure of the cells,the PRV gene-deleted strains were similar with the PRV Fa strain in adsorbing and penetration, but lower in the multiplication speed and the quantity of the virion.

关 键 词：PRV基因缺失疫苗 细胞感染 形态学 超微结构 

分 类 号：S852.65[农业科学—基础兽医学]