17-β-雌二醇对培养的绵羊输卵管上皮细胞β-防御素(sBD-1)mRNA表达的影响  

Impact of 17-β-estradiol on the expression of β-defensin(sBD-1) in epithelial cells of fallopian tubes in the sheep

作　　者：唐博[1,2] 李子义[1] 锡林高娃[2] 付本懂[1] 曹贵方[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]内蒙古农业大学动物科学与医学学院,内蒙古呼和浩特010018

出　　处：《中国兽医学报》2009年第8期1076-1080,共5页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30460093)

摘　　要：建立蒙古绵羊输卵管上皮细胞培养体系作为体外试验模型,根据体内雌激素浓度确定17-β-雌二醇添加的浓度分别为10-8、10-9、10-10、10-11mol/L,提取细胞总RNA,利用实时荧光定量RT-PCR测定sBD-1-mRNA的相对表达量。结果显示:一定浓度范围内(10-8、10-9、10-10mol/L),17-β-雌二醇对培养的输卵管上皮细胞sBD-1-mRNA的表达有促进作用,且呈正相关。结果表明:雌性生理周期下,雌性生殖道sBD-1-mRNA的表达与雌激素相关。To confirm the relationship between 17-β-estradiol and the expression of sBD-1 mRNA in female reproductive tract of Mongolian sheep, the epithelial cell of the fallopian tubes of sheep was established as in vitro model. Based on the concentration of estrogen of sheep in vivo, different concentrations of 17-β-estradiol （10^-8, 10^-9 , 10^-10 and 10^-11 mol/L） were added, following by extraction of the total RNA from cells, and the relative ex- pressions of sBD-1 mRNA were obtained using real time RT-PCR. The results showed that 17-β-estradiol had a positive impact on promoting the relative expression of sBD-1 mRNA in the epithelial cell of the fallopian tubes within the indicated concentrations （10^- 8, 10^-9 and 10 ^-10 mol/L）. These results illuminated the expression of β-defensin was related to estrogen in female reproductive tract.

关 键 词：Β-防御素 实时定量PCR 17-Β-雌二醇 相对表达量 

分 类 号：S852.1[农业科学—基础兽医学]