橡胶树内生真菌分离方法研究  

Separation of Endophytic Fungi in Hevea brasiliensis

作　　者：李晓娜[1] 王赟[1] 李增平[1] 郑服丛[1] 

机构地区：[1]海南大学环境与植物保护学院,海南儋州571737

出　　处：《热带农业科学》2009年第6期5-8,共4页Chinese Journal of Tropical Agriculture

基　　金：海南大学科技基金项目(No.Rnd0719)

摘　　要：以同一株橡胶树的枝条和叶片为材料,采用5种不同的组织表面消毒方法,在加入3种不同细菌生长抑制剂的PDA培养基中培养橡胶树内生真菌。结果表明,在加入Rifamicin 15mg/L的PDA培养基中,运用M2培养分离效果最佳。Leaves and branches from the same rubber tree （Hevea brasiliensis） were collected as materials and sterilized with 5 different sterilization techniques, and cultured in PAD culture medium supplemented with 3 different bacterial growth inhibitors to produce endophytic fungi. The results showed that the leaves and branches sterilized with the M2 method （sterilized with 75% ethanol for 1 min, followed by 4% sodium hypochlorite for 4 min and 75% ethanol for 30 see, and rinsed with aqua sterilisa） and cultured on the PDA medium added with Rifamicin （15 mg/L） produced the largest number of endophytic fungi.

关 键 词：橡胶树 内生真菌 分离方法 

分 类 号：S794.1[农业科学—林木遗传育种] Q949.32[农业科学—林学]