水蛭提取液体外对人视网膜色素上皮细胞PAR-1表达的影响  

Influences of leech extracting solution on expression of thrombin receptor-1 in cultured human retinal pigment epithelial cells

作　　者：王禹燕[1] 郑燕林[1] 刘嘉立[1] 左玉霞[1] 

机构地区：[1]成都中医药大学研究生院,中国四川省成都市610075

出　　处：《国际眼科杂志》2009年第8期1453-1455,共3页International Eye Science

基　　金：中国国家自然科学基金资助项目(No.30371796)~~

摘　　要：目的:研究水蛭提取液对人视网膜色素上皮(retinal pigment epichelial,RPE)细胞PAR-1表达的影响。方法:水蛭提取液体外单独或与凝血酶共同作用于人RPE细胞后,用免疫荧光法测定PAR-1的荧光表达。结果:PAR-1的免疫荧光光密度值比较:(1)凝血酶组与正常对照组比较,PAR-1的IOD值下降(1608±675,4036±1134,P<0.01)。(2)单加水蛭提取液组与正常对照组比较,PAR-1的IOD值升高(9998±2374,4036±1134,P<0.01)。(3)凝血酶和水蛭提取液同时加药组与凝血酶组比较,PAR-1的IOD值显著升高(19664±5436,1608±675,P<0.01)。表明水蛭提取液能竞争性抑制凝血酶对PAR-1的活化作用。结论:水蛭提取液能抑制凝血酶诱导的人RPE细胞膜上的PAR-1的活化,阻断PAR-1介导的细胞信号传导。AIM： To research the influences of leech extracting solution on expression of thrombin receptor-1 in cultured human retinal pigment epithelial（RPE） cells. METHODS： After RPE cells were treated by leech extracting solution alone or leech extracting solution with thrombin, flourescence expression of PAR-1 was mea sured by immumofluorescence method. RESULTS： Flourescence integral optical density（ IOD） of PAR-1 displayed that thrombin group： compared with normal control group, IOD decreased （P 〈 0.01 ） ; leech extract group： compared with normal control group, IOD increased （P 〈 0. 01 ）; leech extracting solution with thrombin group： compared with thrombin group, IOD significantly increased （ P〈 0.01 ）. CONCLUSION：Leech extracting solution seems to be a competitive inhibitor of thrombin during PAR-1 activation in hRPE cells. It can inhibit the activation of PAR-1 by thrombin in human RPE cell membrane and PAR-l-mediated cell signal transduction.

关 键 词：视网膜色素上皮细胞 水蛭提取液 凝血酶 凝血酶受体-1 

分 类 号：R285.5[医药卫生—中药学]