中药复方血栓通可抑制血管生成  

Experimental study of compound xue-shuantong in inhibiting angiogenesis

作　　者：罗旭昇[1] 吴星伟[1] 顾青[1] 张红梅[1] 施宇华[1] 仇亚婷[1] 

机构地区：[1]上海交通大学附属第一人民医院眼科,中国上海市200080

出　　处：《国际眼科杂志》2009年第8期1476-1480,共5页International Eye Science

基　　金：中国上海市卫生局中医药重大课题基金资助项目(No.2006ZD01)~~

摘　　要：目的:探讨复方血栓通对血管生成的作用。方法:鸡胚绒毛尿囊膜(chorioallantoic membrane,CAM)法检测复方血栓通对血管生成的影响;采用MTS比色法观察不同浓度的复方血栓通对血管内皮生长因子(vascular endothelial growth factor,VEGF)诱导的ECV-304细胞增殖的影响;Transwell小室检测不同浓度的复方血栓通对ECV-304细胞移行的影响;Matrigel实验检测不同浓度的复方血栓通对ECV-304细胞内皮管腔形成的影响。结果:复方血栓通中、低浓度组能够抑制鸡胚CAM血管生成;MTS比色法显示,1.5625～100g/L复方血栓通对VEGF诱导的ECV-304细胞增殖具有抑制作用,而更低和更高浓度的复方血栓通则无抑制作用;Transwell小室实验显示,复方血栓通为0,3.125,6.25和12.5g/L时ECV-304细胞移行数分别为219±17,183±14,135±13和19±9;Matrigel实验显示,复方血栓通为0,0.390625,0.78125和1.5625g/L时ECV-304细胞内皮管腔形成数分别为26.3±1.2,18.7±1.5,14.7±0.6和2.7±0.6。结论:复方血栓通具有明显抑制血管生成的作用。AIM：To investigate the effect of compound xueshuantong on angiogenesis. METHODS： Chick embryo chorioallantoic membrane （CAM） method was used to assess the effect of compound xueshuantong on angiogenesis. MTS method was used to access the effect of compound xueshuantong at different concentrations on the proliferation of ECV-304 cells induced by vascular endothelial growth factor （VEGF）. Transwell insert was used to assess the effect of compound xueshuantong at different concentrations on the migration of ECV-304 cells. Matrigel was used to assess the effect of compound xueshuantong at different concentrations on the tube formation of ECV-304 cells. RESULTS： Compound xueshuantong at median and low concentrations could inhibit the angiogenesis of chick embryo CAM. The proliferation of ECV-304 cells induced by VEGF was inhibited by compound xueshuantong at concentrations from 1. 5625 to 100g/L, while it wasn＇t inhibited by compound xueshuantong at higher or lower concentrations. When the concentration of compound xueshuantong was 0, 3. 125, 6. 25, and 12. 5 g/L,. cells number that migrated to under the Transwell membrane was 219 ± 17,183 ±14,135 ± 13, and 19 ± 9 respectively. When the concentration of compound xueshuantong was0, 0. 390625, 0. 78125, and 1. 5625 g/L, tubes number formationed in Matrigel was 26.3 ± 1.2,18.7 ± 1.5,14.7 ± 0. 6,and 2.7 ± 0.6, respectively. CONCLUSION. Compound xueshuantong seems to play a notable role in inhibiting angiogenesis.

关 键 词：复方血栓通 血管生成 绒毛尿囊膜 人脐静脉内皮细胞株(ECV-304) 增殖 移行 管腔形成 

分 类 号：R285.5[医药卫生—中药学]