骨髓基质干细胞软骨化诱导过程中细胞外基质成分的动态性变化  

Chondrogenic induction of bone marrow stem cells: a dynamic process of extra-cellular matrix production

作　　者：张路[1] 李琼[1] 马晓荣[1] 周广东[1] 刘伟[1] 曹谊林[1] 

机构地区：[1]上海第九人民医院整形外科上海组织工程研究重点实验室,上海200011

出　　处：《中国美容医学》2009年第8期1110-1112,共3页Chinese Journal of Aesthetic Medicine

基　　金：国家863重大专项(2006AA02A126);国家自然科学基金项目(30300353);国家973计划项目(2005CB522702)

摘　　要：目的:根据软骨细胞外基质的成分不同,将软骨分为透明软骨、弹性软骨和纤维软骨三种类型。用骨髓基质细胞构建的软骨组织到底是哪一种类型或者更偏向于哪一种类型的软骨是一直被问及的问题。方法:本实验利用组织学的方法对用羊骨髓基质干细胞体外构建的不同时间点的组织工程化软骨进行了分类和评价。结果:经组织学染色发现:在诱导的初期(第一周)组织更偏向于纤维软骨,而从第二周开始组织逐渐向透明软骨转化,至第4周逐渐形成成熟的透明软骨样组织。结论:骨髓基质干细胞在软骨形成过程中细胞外基质呈动态性变化。Objective Cartilage can be divided into three types： hyaline cartilage, elastic cartilage and fibrocartilage, according to different proportion of extra-cellular matrix. The issue about the type of tissue engineered cartilage from Bone Marrow Mesenchymal Stem Cells （BMSCs） is always be posted. Methods The tissue engineered cartilage from BMSCs of different time point were evaluated and classified by means of histochemistry and immunohistology. Results The tissue engineered tissue from 1 week group is more like fibrocartilage. From 2 week group,the tissue gradually transfer into hyaline cartilage and form more mature hyaline like cartilage in 4 weeks group. Conclusions The extracellular matrix of BMSCs shows a dynamic process during chondrogenic induction.

关 键 词：骨髓基质干细胞 软骨诱导 软骨类型 细胞外基质 

分 类 号：Q813.1[生物学—生物工程]