利塞膦酸盐对BMSCs诱导的成骨细胞在脱钙骨支架上生物学行为的影响  

Risedronate Sodium effect of the osteogenesis induced by bone mesenchymal stem cells on decalcification bone scaffolds

作　　者：娄鸣[1] 李晓红[2] 饶国洲[2] 朱勇[1] 方远鹏 左艳萍[1] 王湘[1] 

机构地区：[1]西安医学院口腔系,陕西西安710021 [2]西安交通大学口腔医院修复科,陕西西安710004

出　　处：《中国美容医学》2009年第8期1115-1117,共3页Chinese Journal of Aesthetic Medicine

基　　金：西安医学院科研基金(编号:047);资金名称:利塞膦酸盐对脱钙骨表面黏附成骨细胞诱导骨形成的实验研究

摘　　要：目的:观察不同浓度利塞膦酸盐对脱钙骨支架中成骨细胞活性的影响。方法:选用含不同浓度利塞膦酸钠的培养液(10-7、10-8、10-9、10-10mol/L)对BMSCs诱导的成骨细胞与脱钙骨支架复合物进行培养,通过MTT、上清液碱性磷酸酶活性及I型前胶原羧端肽、骨钙素检测对比观察不同浓度利塞膦酸钠对成骨细胞的作用效果。结果:MTT显示不同浓度利塞膦酸钠组细胞均随培养时间的增加而明显增殖,相同培养时间各不同浓度的利塞膦酸钠组的细胞增殖能力均高于对照组(P<0.05),且在10-10～10-7mol/L浓度范围内呈逐渐增加趋势。各不同浓度的利塞膦酸钠组的上清液ALP活性表达、I型前胶原羧端肽表达、骨钙素表达均随培养时间的增加而明显增加,相同培养时间各组数值均高于对照组(P<0.05),且在10-10～10-7mol/L浓度范围内呈逐渐增加趋势。结论:利塞膦酸钠对成骨细胞在支架材料中的粘附、生长、增殖及骨化作用均有良好的促进作用。Objiective To observe the effects on osteoblasts activity and osteogenesis ability by applying different concentration of Risedronate Sodium on the osteoblasts-decalcification bone scaffolds complex. Methods Co-culturing the cell-scaffold complex under different concentration of Risedronate Sodium （10^-7mol/L, 10^-8mol/L, 10^-9mol/L, 10^-10mol/ L）and observing its effect on the osteoblasts by detecting MTT, ALP, PICP and Osteocalcin. Results MTT showed the tial group has proliferated much more significantly than the control group （P〈0.05）which went up with the concentration levels. ALP, PICP （ Procollagen type Ⅰ C-terminal peptide） and Osteocalcin expression level were much higher in trial group which went up with the concentration levels （P〈0.05）. Conclusion Risedronate Sodium could promote igration, proliferation,differenitiation and calcification of osteoblasts in the scaffold well.

关 键 词：利塞膦酸盐 脱钙骨支架 组织工程 

分 类 号：Q813.1[生物学—生物工程] R318.5[医药卫生—生物医学工程]