从富含多糖的石斛兰花蕾中提取总RNA初探  

Preliminary study on extracting total RNA from polysaccharides-rich Dendrobium buds

作　　者：岑鹏[1] 潘丽晶[1] 张妙彬[1] 范干群[1] 程萍[1] 

机构地区：[1]珠海市农业科学研究中心,广东珠海519070

出　　处：《广东农业科学》2009年第6期162-164,共3页Guangdong Agricultural Sciences

基　　金：广东省科技计划项目(2006B20130004)

摘　　要：石斛兰花蕾中含有大量多糖,干扰RNA的提取与纯化。研究表明,采用含高盐的1.5%SDS裂解液,配合LiCl沉淀RNA,去除多糖类物质,获得了完整性好、质量高的RNA,完全适用于后续的分子生物学研究。Buds of Dendrobium contains high levels of polysaccharides which easily combined with RNA, so it was difficult to extract RNA from buds of Dendrobium. In this research, using 1.5%SDS with high salt concentration, the RNA of Dendrobium buds was precipitated by LiCl in order to get rid of polysaccharide. With this method, high quality RNA was get and thus was worth further biological research.

关 键 词：石斛兰花蕾 多糖 RNA提取 

分 类 号：Q949.718.43[生物学—植物学] Q522