小麦新品种济麦22抗白粉病基因的分子标记定位  

Molecular Mapping of Powdery Mildew Resistance Gene in Wheat Cultivar Jimai 22

作　　者：殷贵鸿[1,2,3] 李根英[4] 何中虎[2,5] 刘建军[4] 王辉[1] 夏先春[2] 

机构地区：[1]西北农林科技大学农学院,陕西杨凌712100 [2]中国农业科学院作物科学研究所/国家小麦改良中心/高家农作物基因资源与基因改良重大科学工程,北京100081 [3]周口市农业科学院,河南周口466001 [4]山东省农业科学院作物研究所,山东济南250100 [5]CIMMYT中国办事处,北京100081

出　　处：《作物学报》2009年第8期1425-1431,共7页Acta Agronomica Sinica

基　　金：国家高技术研究发展计划(863计划)项目(2006AA10Z1A7,2006AA100102);国家自然科学基金项目(30810214)资助

摘　　要：为明确济麦22携带抗白粉病基因的染色体位置,利用济麦22与感病亲本中国春杂交,用小麦白粉菌(Blumeria graminisf.sp.tritici)强毒性小种E20对F2抗、感分离群体和F2:3家系进行抗病鉴定和遗传分析。结果表明,济麦22携带1个显性抗白粉病基因,暂被命名为PmJM22。运用SSR和EST标记及分离群体分组分析法(bulked segregant analysis,BSA),将其定位在2BL染色体上,与4个SSR和5个EST标记间的连锁距离为7.7cM(Xwmc149)到31.3cM(Xbarc101)。通过分析2BL上其他抗白粉病基因的来源、染色体位置和抗性反应,认为PmJM22不同于Pm6、Pm26、Pm33和MlZec1。Wheatpowdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important diseases of wheat （Triticum aestivum L.） worldwide. Breeding resistant wheat cultivars is the most economical and effective approach to control the disease. Jimai 22, a newly released wheat cultivar with high yield, broad adaptability, and good quality, is related to broad-sprectrum resistance to the isolates of B. graminis f. sp. tritici at both seedling and adult plant stages. To map the resistance gene of Jimai 22 on wheat chromosome, we used a highly virulent isolate E20 to screen the F2 plants and F2：3 lines derived from the cross of Jimai 22/Chinese Spring. Genetic analysis indicated that Jimai 22 carried a single dominant genefor resistance to powdery mildew, designated PmJM22 tentatively. Using bulked segregant analysis （BSA） with SSR and STS markers, PmJM22 was located to chromosome 2BL. Linkage analysis indicated that the resistance gene was linked to four SSR and five EST markers, with genetic distances from 7.7 （Xwmc149） to 31.3 cM （Xbarc101）.Based on the origins, chromosome locations, and reaction patterns, PmJM22 is different from all the known powdery mildew resistance genes Pm6, Pm26, Pm33, and Mlzec1 on chromosome 2BL.

关 键 词：普通小麦 白粉病 抗病基因 分子标记 

分 类 号：S512.1[农业科学—作物学]