人类红细胞蛋白激酶C活性变化对CD44分子表达及其亚细胞分布的影响  

Effects of Protein Kinase C Activity on CD44 Expression and Subcellular Distribution in Human Erythrocytes

作　　者：郝一文[1] 程大也[1] 陈进涛[1] 

机构地区：[1]中国医科大学附属第一医院输血科,辽宁沈阳110001

出　　处：《中国实验血液学杂志》2009年第4期933-937,共5页Journal of Experimental Hematology

基　　金：辽宁省教育厅科研基金资助项目;编号05L515

摘　　要：本研究旨在探讨人类红细胞蛋白激酶C(protein kinase C,PKC)活性对标准型CD44分子表达及其亚细胞分布的影响。通过外源性底物对[γ-32P]-ATP的摄入量测定红细胞蛋白激酶C的活性;通过放射自显影法测定CD44分子的磷酸化;采用间接免疫荧光法观察CD44亚细胞分布;采用流式分析法测定CD44分子的表达。结果显示:PKC激活剂佛波酯(phorbol-myristate acetate,PMA)处理人红细胞30分钟时PKC活性达高峰,此时CD44的磷酸化水平最高,CD44分子表达也达到了峰值,与对照组比较差异有统计学意义(p<0.001)。PKC活化导致CD44在细胞膜上聚集,而PKC则聚集在CD44处。Calphostin C能抑制PKC的上述作用。结论:PKC活化通过磷酸化作用上调CD44分子的表达,并导致CD44与PKC的同步移位与共分布。The study was aimed to investigate the effects of protein kinase C （ PKC ） on standard type CD44 expression and subcellular distribution in human erythrocytes. PKC activity was detected by the incorporation of [ γ-^32p]-ATP into exogenous substrate, phosphorylation of CD44 was detemined by autoradiograph, distribution of CD44 was observed by indirect immunofluorescence, and expression of CD44 was analyzed by flow cytometry. The results showed that PKC activity reached the maximal level at 30 minutes after treatment with phorbol-myristate-acetate（ PMA）, and the peak of CD44 phosphorylation and CD44 expression appeared at the same time, which all increased significantly as compared with control group （p 〈0. 001 ）. PKC activation resulted in CD44 aggregation on membrane and colocalization of PKC and CD44. Calphostin C could inhibit the above reaction resulted from PKC activation. It is concluded that PKC activation can up-regulate CD44 expression by phosphorylation, and result in the coherent migration and colocalization of CD44 and PKC in human erythrocytes.

关 键 词：红细胞 蛋白激酶C CD44 

分 类 号：R331.141[医药卫生—人体生理学] R392[医药卫生—基础医学]