STR-PCR和FISH在监测异性别异基因造血干细胞移植后嵌合状态中意义的比较  

Comparison of STR-PCR and FISH Value for Monitoring Chimerism after Sex-mismatched Allogeneic Hematopoietic Stem Cell Transplantation

作　　者：秦尤文[1] 蒋瑛[1] 王小蕊[1] 万理萍[1] 颜式可[1] 王椿[1] 

机构地区：[1]上海交通大学附属第一人民医院血液科,上海200080

出　　处：《中国实验血液学杂志》2009年第4期1016-1020,共5页Journal of Experimental Hematology

摘　　要：本研究比较短串联重复序列PCR(STR-PCR)和荧光原位杂交(FISH)两种方法在异基因造血干细胞移植后血细胞嵌合状态监测中的意义。对38例接受异性别异基因造血干细胞移植的患者,采用STR-PCR和FISH定量测定移植后14、28天和3个月的骨髓或外周血细胞嵌合状态。结果表明,14天时STR-PCR和FISH同时检测的30例中分别有14例和8例达到完全嵌合(CC,p>0.05)。28天时两者同时检测的31例中分别有26例和15例达到CC(p<0.01)。3个月时两种方法共同监测的24例中分别有22例和17例达到CC(p>0.05)。连续监测3个月以上的28例患者中14例发生移植物被排斥或复发,其中9例由FISH首先检出(p<0.05)。结论:FISH检测比STR-PCR更为准确地反映移植早期造血细胞嵌合状态,有助于监测移植物被排斥和疾病复发。This study was purposed to compare the significance of multiplex short tandem repeat polymerase chain reaction （STR-PCR） and fluorescent in situ hybridization （FISH） for monitoring chimerism after sex-mismatched allogeneic hematopoietic stem cell transplantation （allo-HSCT）. The chimerism of bone marrow or peripheral blood cells from 38 patients was analyzed by STR-PCR and FISH on days 14, 28 and at 3 months after alIo-HSCT. The results indicated that on day 14, the complete chimerism （CC） was detected in 14 of 30 cases by STR-PCR and in 8 of 30 cases by FISH （p 〉0.05）. On day 28, the CC was detected in 26 of 31 cases by STR-PCR and in 15 of 31 cases by FISH （p 〈0.01 ）. At 3 months, the CC was observed in 22 of 24 cases by STR-PCR and 17 of 24 cases by FISH （p 〉0.05）. 14 cases were found to have a graft rejection or relapse among 28 cases which were continuously monitored more than 3 months post the transplants. Donor cell decrease in 9 of 14 cases was proved by FISH alone. It is concluded that FISH is more sensitive than STR-PCR in early monitoring chimerism status of post-transplant and in predicting graft rejection or disease relapse.

关 键 词：异性别异基因造血干细胞移植 短串联重复序列 荧光原位杂交 

分 类 号：Q503[生物学—生物化学] R457.7[医药卫生—治疗学]