心灵丸中三七皂苷R_1、人参皂苷R_(g1)和R_(b1)的含量测定  

Determination of Notoginsenoside R_1 and Ginsenoside R_(g1),R_(b1) in Xinling Pills by HPLC

作　　者：林晓辉 张雪原 林史珍[2] 朱吉士 

机构地区：[1]广东省潮州市药品检验所,广东潮州521000 [2]华南农业大学,广东广州510642

出　　处：《中国药业》2009年第16期27-28,共2页China Pharmaceuticals

摘　　要：目的建立同时测定心灵丸中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1含量的高效液相色谱(HPLC)法。方法色谱柱为Agilent Hypersil ODS柱(250mm×4.0mm,5μm),流动相A为乙腈,B为水,梯度洗脱(0min19%A→12min19%A→60min36%A),流速为1.0mL/min,检测波长为203nm。结果三七皂苷R1进样量在0.1378～2.7560μg范围内与峰面积线性关系良好,r=0.9997,平均回收率为98.95%,RSD为0.67%;人参皂苷Rg1进样量在0.5672～11.3440μg范围内与峰面积线性关系良好,r=0.9999,平均回收率为99.10%,RSD为0.29%;人参皂苷Rb1进样量在0.4186～8.3720μg范围内与峰面积线性关系良好,r=0.9999,平均回收率为99.02%,RSD为0.42%。结论HPLC法简便准确、专属性强,可用于心灵丸的质量控制。Objective To establish a HPLC method for the content determination of notoginsenoside R1 and ginsenoside Rg1, Rb1 in Xinling Pills. Methods The analysis was performed on the Agilent Hypersil ODS column（250 mm × 4.0 mm,5 μm）, using the mixture of acetonitrile （A） and water（B） as the mobile phase. Elution was performed using the gradient mode（0 min 19% A→12 min 19% A→60 rain 36% A）, and the flow rate was 1.0 mL/min. The detection wavelength was 203 nm. Results There was good linearity in the range of 0. 137 8- 2. 756 0 μg for notoginsenoside R1 （ r = 0. 999 7）, 0. 567 2 - 11. 344 0 μg for ginsenoside Rg1 （ r = 0. 999 9） and 0. 418 6 - 8. 372 0 μg for ginsenoside Rb1（r=0. 999 9）.The average recovery rates and RSD were 98.95% and 0. 67% for notoginsenoside R1, 99.10% and 0. 29% for ginsenoside Rg1 and 99.02% and 0. 42% for ginsenoside Rb1. Conclusion This method is simple and reliable for the quality control of Xinling Pills.

关 键 词：心灵丸 三七皂苷R1 人参皂苷RG1 人参皂苷RB1 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]