高效液相色谱法测定水杨酸醇溶液中水杨酸含量  

Determination of Content of Salicylic Acid in Salicylic Acid Alcoholic Solution by HPLC

作　　者：周蓓瀛[1] 李楠[2] 张钢平[3] 李莉[3] 郄冰冰[4] 

机构地区：[1]冀中能源峰峰集团总医院药剂科,河北邯郸056200 [2]河北省邯郸市铁路医院,河北邯郸056000 [3]河北省邯郸市药品检验所,河北邯郸056001 [4]河北省药品检验所,河北石家庄050011

出　　处：《中国药业》2009年第16期38-39,共2页China Pharmaceuticals

摘　　要：目的采用高效液相色谱(HPLC)法测定水杨酸醇溶液中水杨酸的含量。方法以Eclipse XDB-C18柱(250mm×4.6mm,5μm)为色谱柱,以甲醇-0.4%磷酸溶液(58∶42)为流动相,检测波长为298nm,流速为1.0mL/min,柱温为25℃。结果水杨酸进样量在0.16～0.80μg范围内与峰面积积分值呈良好的线性关系,r=1.0000(n=6),平均回收率为99.51%,RSD为0.2%(n=6)。结论HPLC法简单、结果准确、灵敏度高,可用于水杨酸醇溶液中水杨酸的含量测定。Objective To develop a HPLC method for determining the content of salicylic acid in Salicylic Acid Alcoholic Solution. Methods The determination was performed on the Eclipse XDB C18 column, using methylalcohol-0. 4% phosphoric acid solution（58：42）as the mobile phase at the flow rate of 1.0 mL/min and the detection wavelength at 298 nm. The column temperature was 25 ℃. Results The linear relation of the peak area of salicylic acid was determined as 0.16-0.80 μg by HPLC with the regression coefficient of 1. 000 0 and the average recovery rate of 99.51%, RSD = 0. 2% （n = 6）. Conclusion The method is simple, rapid and accurate. It can be used for determining salicylic acid in Salicylic Acid Alcoholic Solution.

关 键 词：水杨酸醇溶液 水杨酸 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学] R986