酶学检测精氨琥珀酸酶方法的建立  

Establish an automatic analysis method for the determination of serum argininosuccinatelyase

作　　者：陆建荣[1] 罗文明[1] 吴月平[1] 毛丽萍[1] 常秋月 

机构地区：[1]江苏省南通市第三人民医院检验科,226006 [2]江苏省南通市中心血站,226006

出　　处：《检验医学与临床》2009年第16期1345-1346,共2页Laboratory Medicine and Clinic

摘　　要：目的建立酶学检测精氨琥珀酸酶(ASAL)的方法。方法精氨琥珀酸在ASAL的作用下生成精氨酸和延胡索酸,后者被延胡索酸酶催化生成苹果酸,苹果酸被苹果酸脱氢酶催化生成草酰乙酸,同时NAD+(烟酰胺腺嘌呤二核苷酸,即辅酶Ⅰ)还原为NADH(还原型烟酰胺腺嘌呤二核苷酸)。pH9.0的肼-甘氨酸缓冲液使反应完全。结果ASAL回收率为97%,在0～87U/L内为线性;批内变异系数(CV)为5.3%,天间CV为6.5%;改良酶学检测方法对255μmol/L胆红素无影响;对0.1g/L血红蛋白有轻度影响。结论所建立的酶学检测方法结果准确可靠,易自动化。Objective To establish an automatic analysis method for the determination of serum argininosuecinatelyase （ASAL）, Methods First argininosuccinic acid was cataloged to arginin fumarate by argininosuecinatelyase, and then the fumarate was cataloged to malicacid by fumarase, the malicacid was then cataloged to oxaloacetate by malate dehydrogensae with the simultaneous production of NADH from NAD^＋. We added hydrazine to the reaction mixture to remove oxaloacetate which allowed the reaction to proceed to completion. Results The rate of the recoveries were 97% . The reaction was linear up to 87U/L. The mean within day and between day imprecision（cv） was 5.3% and 6.5% respectively. No interference by 255umol/Lbilirubin or 0.1 g/L hemoglobin was observed. Conclusion This method is accurate, reliable for serum argininosuccinatelyase analysis and is amenable to automation.

关 键 词：精氨琥珀酸酶 延胡索酸水合酶 苹果酸脱氢酶 自动分析 方法 

分 类 号：R345.5[医药卫生—基础医学] R446.112