一口钟提取物治疗大鼠佐剂性关节炎研究  

Effects of Yikouzhong Extracts on Adjuvant Arthritis in Rats

作　　者：陈芳芳[1] 沈楠[1] 雷钧涛[1] 丁肖梁[1] 李永亮[1] 刘斌[1] 郝程杰[1] 王媛[1] 张秀荣[1] 

机构地区：[1]吉林医药学院,132013

出　　处：《医药导报》2009年第8期990-992,共3页Herald of Medicine

摘　　要：目的探讨一口钟不同溶剂提取物对大鼠佐剂性关节炎的治疗作用。方法采用不同有机溶剂对一口钟进行提取,得到各溶剂的提取物。将70只雄性Wistar大鼠随机分为正常组、模型组、石油醚组、三氯甲烷组、乙酸乙酯组、95%乙醇组和5%乙醇组各10只;正常组大鼠左后足跖皮内注射0.9%氯化钠注射液,每只0.1mL;其他各组大鼠左后足跖皮内注射弗氏完全佐剂,每只0.1mL。实验第10天,正常组和模型组给予0.9%氯化钠注射液灌胃,其他各组分别灌胃给予一口钟不同溶剂提取物,均0.5mL·(200g)-1,连续16d。检测各组大鼠足跖肿胀、脾脏指数、胸腺指数及血清一氧化氮含量。结果除一口钟石油醚提取物组外,与模型组比较,其他各提取物均能显著抑制大鼠左后足跖肿胀;一口钟不同溶剂提取物组大鼠胸腺指数、脾脏指数均显著增高,血清一氧化氮浓度显著降低。结论一口钟提取物对大鼠佐剂性关节炎有显著治疗作用,推测一口钟治疗佐剂性关节炎有效部位为脂溶性成分。Objective To explore effects of different solvent extractions of yikouzhong（fruit of eycalptus globlus） on rat adjuvant arthritis. Methods Different extracts were obtained from yikouzhong with different organic solvents. 70 Female Wistar rats were randomly divided into normal group （saline）, model group, petroleum ether group, chloroform group, acetoacetate group, 95% ethanol group and 50% ehanol . Adjuvant arthritis （AA） in rats was established by intradermal injection with Freund＇s complete adjuvant （0.1 mL / one） in Wistar rats, while 0.9% sodium chloride as normal control. The rats in normal and model controls were treated with normal saline, others with different extracts, all at 0.5 mL · （200 g） ^-1, for consecutive 16 d. Swelling in foot pads, spleen index, thymus index as well as levels of nitric oxide （NO） in sera were determined. Results Compared with model group, all extracts significantly inhibited foot pad swelling, but petroleum group. Meanwhile, the thymus index, spleen index and serum concentrations of NO were markedly reduced by different solvent extracts. Conclusion Extracts from yikouzhong has obvious therapeutic effect on rat adjuvant arthritis, the active components of which is supposed to be liposoluble.

关 键 词：一口钟 类风湿关节炎 佐剂性关节炎 

分 类 号：R286[医药卫生—中药学] R965[医药卫生—中医学]