奈替米星和1，2-萘醌-4-磺酸钠显色反应的研究及对奈替米星的测定  

Study on the Chromogenic Reaction of Netilmicin with Sodium 1,2-Naphthoquinone-4-sulfonate and the Determination of Netilmicin by Spectrophotometry

作　　者：吴云玲[1] 占海红[2] 李晶[1] 李全民[1] 

机构地区：[1]河南师范大学化学与环境科学学院,河南省环境污染控制重点实验室,河南新乡453007 [2]平顶山教育学院生化系,河南平顶山467000

出　　处：《分析科学学报》2009年第4期431-434,共4页Journal of Analytical Science

基　　金：河南省教育厅自然科学基金(No.2008B150011)

摘　　要：建立了用1，2-萘醌-4-磺酸钠（NQS）作显色剂测定奈替米星（NET）的新方法。研究表明：控制溶液pH-10．00，NET与NQS反应生成摩尔比为1：3的红褐色产物，最大吸收波长λmax=445nm，奈替米星浓度在6．4～57．6mg／L范围内与吸光度呈良好线性关系，表观摩尔吸光系数ε=6．31×10。L·mol^-1·cm^-1，方法检出限为5．9mg／L，相对标准偏差（RSD）为0．81％，平均回收率99．0％以上。该法能直接用于药物样品中奈替米星的测定，结果满意。A new method for the determination of netilmicin （NET） by spectrophotometry with 1,2- naphthoquinone-4-sulfonate（NQS） as a chromogenic reagent has been developed in this paper. The study indicated that the molar ratio of the russety product formed by the reaction between NET and NQS was 1：3 at pH=10.00, and ε was 6. 64×103 L·mo1^-1·cm^-1 at 445 nm. The equation of the linear regression could be presented as A=0. 066＋4.1 × 10^-3 c（mg/L）, with a linear correlation coefficient of 0. 9953. Beer＇s law was well obeyed in the range of NET concentrations of 6. 4 - 57. 6 mg/L. The average recovery was over 99. 0%. The method was used for the determination of NET in pharmaceutical samples successfully.

关 键 词：分光光度法 奈替米星 1 2-萘醌-4-磺酸钠 

分 类 号：O657.3[理学—分析化学]