改良Trizol法提取香蕉叶片总RNA  

Extraction of total RNA from banana with improved Trizol method

作　　者：孙德权[1,2] 郭启高[1] 胡玉林[2] 谢江辉[2] 

机构地区：[1]西南大学园艺园林学院,重庆400716 [2]中国热带农业科学院南亚热带作物研究所,广东湛江524091

出　　处：《广东农业科学》2009年第5期162-164,共3页Guangdong Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务费专项(SSRI200714);海南省自然科学基金资助项目(39870532)

摘　　要：针对香蕉组织中多糖、多酚等次生物质含量高的特点,采用改良Trizol法,成功地从香蕉叶片中提取了总RNA。所提取的总RNA A260/A280和A260/A230值分别为1.91和2.10。电泳检测显示28 s、18 s条带完整清晰,以此RNA为模板进行RT-PCR,结果成功得到目标特异条带。试验结果表明:改良Trizol法具有方便快捷、完整性好、受多糖多酚影响较少以及无DNA和蛋白质污染等优点,对具有香蕉叶片类似成分的其他作物的RNA提取具有一定的借鉴意义。The total RNA was extracted from mature banana leaf which had plenty of polysaccharides and hydroxybezene with improved Trizol method. The electrophoresis pattern showed the bands of 28 s rRNA and lgs rRNA were sharp and complete, A260/A280and A260/A230 values of the extracted RNA were 1.91 and 2.10. The result of RT-PCR with the total RNA was positive. RNA with high quality and high quanity can be extracted with this method from plant materials which have plenty of polysaccharides and hydroxybezene.

关 键 词：香蕉 总RNA提取 改良Trizol法 

分 类 号：Q522[生物学—生物化学] Q943.2