鸭疫里默氏杆菌原生质体制备与再生的研究  

Studies on protoplast formation and regeneration of Riemerella anatipestifer strain

作　　者：王春平[1,2] 韦强[2] 崔言顺[1] 鲍国连[2] 刘燕[2] 邵泽香[1,2] 

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]浙江省农业科学院畜牧兽医研究所,浙江杭州310021

出　　处：《浙江农业学报》2009年第4期311-315,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省重点科技攻关项目(2005C12021);浙江省农科院创新能力提升工程项目

摘　　要：以耐药标记的鸭疫里默氏杆菌TXRA1(Chlr,Erys)为亲本菌株,在DNase I始终存在的条件下,采用Lysozyme-EDTA法制备原生质体,然后在高渗琼脂平板上再生。系统地优化了影响原生质体制备及再生的各种条件,摸索出鸭疫里默氏杆菌TXRA1以Lysozyme 0.1 g/L作用20 min后补加EDTA至终浓度为0.01 mol/L继续作用45 min为最佳试验条件,并成功获得了92%的原生质体制备率和38.77%的再生率。Using Riemerella anatipestifer TXRA1 （Chl^Γ, Ery^S） strain with drug resistance label as the parental strain, protoplasts were prepared with lysozyme and EDTA at the presence of DNase I, then the protoplasts were regenerated on hypertonic medium. Conditions of protoplast formation and regeneration were optimized, indicating that the optimum Riemerella anatipestifer TXRA1（Chl^Γ,Ery^S） added with 0.1 g/L lysozyme and reacted for 20 min and then EDTA was added slowly to a final concentration of 0.01 mol/L and reacted for another 45min, in which the protoplast formation rate was 92% and the regeneration rate was 38.77%.

关 键 词：鸭疫里默氏杆菌 原生质体 制备 再生 

分 类 号：S852.65[农业科学—基础兽医学]