芥蓝查尔酮合成酶基因BaCHS的克隆与序列分析  

Isolation and sequence analysis of chalcone synthase gene BaCHS from Brassica albograbra

作　　者：蒋明[1] 苗立祥[2] 胡齐赞[3] 贺蔡明[1] 陈珍[1] 

机构地区：[1]台州学院生命科学学院,浙江临海317000 [2]浙江省农业科学院园艺研究所,浙江杭州310021 [3]浙江省农业科学院蔬菜研究所,浙江杭州310021

出　　处：《浙江农业学报》2009年第4期321-326,共6页Acta Agriculturae Zhejiangensis

基　　金：浙江省自然科学基金项目(Y3080081);台州市科技计划项目(08XH02)

摘　　要：查尔酮合成酶基因是类黄酮生物合成途径的关键基因,在植物发育和逆境反应中起着重要作用。根据已发表的查尔酮合成酶基因序列设计全长引物,以芥蓝(Brassica albograbra)叶片基因组DNA和花蕾cD-NA为模板,克隆到了芥蓝查尔酮合成酶基因全长序列,命名为BaCHS。该基因DNA全长为1 263 bp,具一个长度为75 bp的内含子,编码区长度为1 188 bp,编码395个氨基酸。序列比对结果表明,BaCHS基因编码的氨基酸序列与其他十字花科植物之间的同源性很高,仅存在个别氨基酸残基的差别。Chalcone synthase gene is a key gene in flavonoid biosynthetic pathway and plays important roles in plant development and stress response. Full length primer pairs were designed according to known chalcone synthase gene sequence. The gene, designated BACHS, was isolated successhtUy from leaf genomic DNA and fower bud cDNA of Chinese kale （ Brassica albograbra）, respectively. Genomic DNA of BACHS gene is 1 263 bp in length with one intro of 75 bp. Complete coding sequence of BACHS is 1 188 bp in length encoding 395 amino acids. Protein sequence comparison showed that BACHS shared high homology with those of crucifer plants and there were only few differences in amino acid residues.

关 键 词：芥蓝 查尔酮合成酶 序列分析 

分 类 号：Q78[生物学—分子生物学]