急性热应激对蛋鸭内脏组织HSP70表达的影响  

Effects of acute heat stress on expressional patterns of the heat shock protein 70 in the duck internal organs

作　　者：邹丽丽[1,2] 陶争荣[2] 沈军达[2] 任晋东[2,3] 石放雄[1] 卢立志[2] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095 [2]浙江省农业科学院畜牧兽医研究所,浙江杭州310021 [3]西北农林科技大学动物科技学院,陕西杨凌710021

出　　处：《浙江农业学报》2009年第4期332-334,共3页Acta Agriculturae Zhejiangensis

基　　金：国家现代水禽产业技术体系建设专项;国家"十一五"科技支撑计划课题(2006BAD14B06);浙江省重大科技攻关项目(2005C12005-01);浙江省重大国际合作项目(2006C14015)

摘　　要：选取20只处于产蛋高峰期的蛋鸭,急性热应激处理后,用RT-PCR和Western-blot研究蛋鸭各内脏器官内热休克蛋白70(heat shock protein 70,HSP70)的表达情况。结果显示:急性热应激后内脏组织肝脏、心脏和卵巢中的HSP70 mRNA和蛋白水平的表达量无明显变化,肾脏内mRNA水平的表达量显著下降,而脾脏内mRNA和蛋白水平的表达量呈短暂下降后又恢复到原来水平。结果表明当蛋鸭遭受急性热应激时内脏器官呈现特异性反应。Twenty laying ducks were exposed under acute heat stress during the peak period of egg-laying, and the expressional patterns of heat shock protein 70 （HSP70） in the internal organs of these ducks were examined using RT - PCR and western-blot. The result indicated that the mRNA and protein levels of HSP70 in heart, liver and ovary were not changed after acute heat stress, while the mRNA levels of HSP70 were reduced in the kidney and the mRNA and protein levels of HSP70 in the spleen were transitorily decreased but recovered after 7 h of acute heat stress. In conclusion, the expression of HSP70 in the internal organs of laying ducks showed specific responses under acute heat stress.

关 键 词：急性热应激 HSP70 蛋鸭 

分 类 号：S858.32[农业科学—临床兽医学]