增强UV-B辐射下一氧化氮对小球藻氮素代谢和类囊体的保护作用  

Effect of nitric oxide on N metabolism and Thylakoid membrane in Chlorella pyrenodosa response to UV-B radiation

作　　者：陈坤[1,2] 朱涛[1] 张娅婷[1] 杨桂芳[1] 刘永定[2] 

机构地区：[1]信阳师范学院生命科学学院,河南信阳464000 [2]中国科学院水生生物研究所,湖北武汉430072

出　　处：《生态环境学报》2009年第3期865-868,共4页Ecology and Environmental Sciences

基　　金：863计划基金项目资助(2002AA601021);国家自然科学基金项目资助(30470276)

摘　　要：由于到达地球表面的紫外线B辐射不断加强,生物生长受到了威胁。UV-B的增强改变了生物体赖以生存的环境,影响了藻类生物生长,抑制了其光合作用。以BG11为培养基,在室内培养的条件是光照强度为60μmol·m-2s-(1昼夜比为12h∶12h),温度为26℃,研究了一氧化氮(NO)在增强UV-B(强度为0.2J·m-2s-1)辐射下的对小球藻的作用。测定了小球藻的硝酸还原酶(NR,nitrate reductase)、亚硝酸还原酶(NiR,nitrite reductase)、谷胱甘肽还原酶(GS,glutamine synthetase)、谷氨酸-草酰乙酸转氨酶(GOT,Glutamine aminotransferase)的活性变化,并对小球球藻进行了显微结构观察。结果显示:在增加UV-B辐射下,一氧化氮能够提高其氮素代谢酶类活性,保护内囊体膜避免UV-B的损伤。结论是UV-B辐射下一氧化氮对小球藻具有保护作用。The enhanced ultraviolet radiation B （UV-B） reaching the earth is increasing. The organisms are faced with new challenge The UV-B radiation increase Cultivated in BG11 medium, changes the biological living environment, influences the growth of algae, inhibits the photosynthesis. temperatures （26 ℃） under light intensties （60 μmol photos·m-2·s-1, 12 h/12 h, light： dark） in doors, it was studied that the effects of nitric oxide in Chlorellapyrenodosa were carried out in UV-B radiation（0.2 J·m2·s-1）. The activities of nitrate reductase, nitrite reductase, glutamine synthetase, glutamine aminotransferase were measured and microstructure of the cell morphy were set out. The results shows that the nitric oxide boosted the activities of enzyme on N metabolism and against damage on thylakoid membrane induced by UV-B radiation. Together this results that nitric oxide plays key role in Chlorella pyrenodosa under enhanced ultraviolet radiation B.

关 键 词：小球藻 一氧化氮 UV-B辐射 

分 类 号：Q945[生物学—植物学]