不同浓度吡咯烷二硫氨基甲酸对高糖下大鼠肾成纤维细胞单核细胞趋化蛋白-1表达的影响  

Effect of different concentrations of PDTC on the expression of monocyte chemotactic protein-1 in rat kidney fibroblasts under high glucose circumstance

作　　者：刘梦江[1] 李竞[1] 王洁敏[1] 

机构地区：[1]武汉大学人民医院内分泌科,430060

出　　处：《实用医学杂志》2009年第16期2617-2619,共3页The Journal of Practical Medicine

基　　金：湖北省科技厅自然基金(编号:2007ABA134)

摘　　要：目的:探讨不同浓度吡咯烷二硫氨基甲酸(PDTC)对高糖下大鼠成纤维细胞(NRK)中单核细胞趋化蛋白-1(MCP-1)表达的影响。方法:体外培养大鼠NRK细胞;葡萄糖孵育下分6组,正常对照组、高糖1组、高糖2组含葡萄糖分别为5.6、15和30mmol/L,干预组在葡萄糖30mmo/L基础上分别加入PDTC5、10和20μmol/L;RT-PCR检测24、48h后各组MCP-1mRNA表达水平,免疫细胞化学(ICC)法检测24、48h各组的MCP-1蛋白表达情况。结果:与正常对照组相比,高糖组MCP-1mRNA和蛋白表达水平显著升高(P<0.05),且呈剂量时间依赖性;而不同浓度PDTC干预后,MCP-1mRNA和蛋白表达水平显著下降(P<0.05),且与PDTC呈剂量时间依赖性。结论:PDTC下调NRK细胞中MCP-1mRNA和蛋白的表达。Objective To explore the effect of different concentrations of PDTC on the expression of monocyte chemotactic protein-1 （MCP-1） in rat kidney fibroblasts （NRK） under high glucose circumstance. Methods NRK were cultured in vitro, and then were assigned to receive 5.6 mmol/L glucose （group 1）, 15 mmol/L glucose （group 2）, 30 mmol/L glucose （group 3）, 30 mmol/L glucose ＋ 5 μmol/L PDTC （group 4）, 30 mmol/L glucose ＋ 10 μmol/L PDTC （group 5）, or 30 mmol/L glucose ＋ 20 μmol/L PDTC （group 6）. The expression of MCP-1 mRNA was measured by RT-PCR and MCP-1 was measured by ICC after 24 h and 48 h, respectively. Results As compared with group 1, MCP-1 mRNA and MCP-1 were remarkably increased in a time-dose dependent manner in group 2 and group 3 （P 〈 0.05）. MCP-1 mRNA and MCP-1 were remarkably decreased in a time-dose dependent manner in group 4, group 5, and group 6, as compared with those in group 3（P 〈 0.05）. Conclusion PTDC can downregulate the expression of MCP-1 mRNA and MCP-1 in NRK.

关 键 词：糖尿病肾病 单核细胞趋化蛋白-1 吡咯烷二硫氨基甲酸 

分 类 号：R587.2[医药卫生—内分泌] R776[医药卫生—内科学]