PCR-RFLP技术检测嘉兴凤桥种猪氟烷基因  

Detection on the Halothane Gene in Fengqiao Breeding Pigs of Jiaxing by PCR-RFLP Technique

作　　者：杜晓婷[1] 刘锐[2] 张水明 杨卫景 吴正红 陆建中 金建芳 

机构地区：[1]浙江工业大学,浙江杭州310019 [2]嘉兴职业技术学院,浙江嘉兴314036 [3]嘉兴凤桥畜牧兽医站,浙江嘉兴314008 [4]嘉兴南湖区畜牧兽医局,浙江嘉兴314000 [5]嘉兴正宏生物科技有限公司,浙江嘉兴314000 [6]嘉兴凤桥农业技术服务中心,浙江嘉兴314008

出　　处：《安徽农业科学》2009年第24期11437-11438,共2页Journal of Anhui Agricultural Sciences

基　　金：嘉兴市科技计划项目(2008BY8008)

摘　　要：[目的]为凤桥镇通过合理利用氟烷基因提高猪肉品质奠定理论基础。[方法]从嘉兴县凤桥镇的规模化养猪场随机抽取种猪血样,通过PCR-RFLP技术进行氟烷基因检测。[结果]HhaⅠ内切酶的识别位点为5′GCG↓C3′。PCR扩增产物是含RYR1基因第1 843位点长度为659 bp的片段。如果猪的基因型为NN,由于未发生胞嘧啶C到胸腺嘧啶T的突变,可以看到2条带。如果猪的基因型为nn,由于发生了胞嘧啶C到胸腺嘧啶T的突变,只能看到1条带。如果猪的基因型为Nn,可以看到3条带。在长白猪、杜洛克猪和大约克猪3个供试品种中没有检测出氟烷基因隐性纯合子(nn),其n等位基因频率分别为2.5%、1.9%和1.6%。[结论]建议在凤桥镇利用的氟烷基因杂合子(Nn)的公系和氟烷基因显性纯合子(NN)的母系开发猪的胴体优势。[ Objective ] The purpose was to lay a theoretical foundation for enhancing pork quality through utilizing halothane gene reasonably in Fengqiao town. [ Method] The blood samples were extracted from breeding pigs randomly in the large-scale piggeries of Fengqiao town in Jiaxing County and the halothane gene was detected by PCR-RFLP technique.[ Result] Tile recognition site of endonuclease Hha I was 5＇GCG↓ C3＇. The PCR amplified product was a segment containing the 1843rd site of gene RYR1 and its length was 659 bp. If the genotype of pig was NN, there would be 2 bands appeared for that there was no mutagenesis from cytosine C to thymine T occurred. If the genotype of pig was nn, there would be only 1 band appeared for that there was mutagenesis from cytosine C to thymine T occurred. If the genotype of pig was Nn,there would be 3 bands appeared. There was no allozygote （nn） of halothane gene detected out in the 3 tested varieties of landrace pig,duroc pig and yorkshire pig and their frequences of allele gene n were 2.5% , 1.9% and 1.6% resp. [ Conclusion] It was suggested that the carcass advantage of pig should be developed by using male lines with heterozygote （Nn） of halothane gene and female lines with dominant homozygote （ NN ） of halothane gene in Fengqiao town.

关 键 词：凤桥 猪 氟烷基因 PCR—RFLP 

分 类 号：S828[农业科学—畜牧学]