近交系小鼠5个品系10个微卫星基因位点多态性分析  

Polymorphism analysis for ten microsatellite points between five strains of mice

作　　者：郭杰[1] 王婷婷[1] 郑振宇[1] 李春丽[1] 赵卫东[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《河南农业大学学报》2009年第3期315-318,共4页Journal of Henan Agricultural University

基　　金：国家自然科学基金项目(30770321);教育部科学技术研究重点项目(206083);河南省教育厅高校杰出科研人才创新工程项目(006KYCX015)

摘　　要：利用PCR扩增对近交系小鼠5个品系的10个微卫星基因位点进行多态性分析.结果表明,在DDK与B6品系之间有多态性的引物2个,实验室品系和野生小鼠品系之间的差异较大.利用这10个微卫星基因位点可以将5个小鼠品系区分开来,尤其是D14Mit6在5种小鼠品系中表现出更明显特异性.Using PCR technology, we analyzed the polymorphism for ten microsatellite points betweem five strains of mice. According to the bands with a comparative analysis, we found polymorphism of two primers between the DDK and Bs strains particularly in the different subspecies of polymorphisms between the laboratory mouse strains and wild mouse-derived strains. Ten micro-satellites of gene point were used to distinguish the five strains of mice. The micro-satellite D14Mit6 in five strains of mice showed particularly significant specificity.

关 键 词：小鼠 微卫星 聚合酶链反应 多态性分析 

分 类 号：Q523[生物学—生物化学]